Grb2 dominantly associates with dynamin II in human hepatocellular carcinoma HepG2 cells

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dc.contributor.authorSun Young Yoon-
dc.contributor.authorMoon Jin Jeong-
dc.contributor.authorJi Yun Yoo-
dc.contributor.authorKyung Im Lee-
dc.contributor.authorByoung-Mog Kwon-
dc.contributor.authorDo Seon Lim-
dc.contributor.authorChoong Eun Lee-
dc.contributor.authorYoung Mee Park-
dc.contributor.authorMi Young Han-
dc.date.accessioned2017-04-19T08:58:41Z-
dc.date.available2017-04-19T08:58:41Z-
dc.date.issued2002-
dc.identifier.issn0730-2312-
dc.identifier.uri10.1002/jcb.1275ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5698-
dc.description.abstractThe two SH3 domains and one SH2 domain containing adaptor protein Grb2 is an essential element of the Ras signaling pathway in multiple systems. The SH2 domain of Grb2 recognizes and interacts with phosphotyrosine residues on activated tyrosine kinases, whereas the SH3 domains bind to several proline-rich domain-containing proteins such as Sos1. To define the difference in Grb2-associated proteins in hepatocarcinoma cells, we performed coprecipitation analysis using recombinant GST-Grb2 fusion proteins and found that several protein components (p170, p125, pl00, and p80) differently associated with GST-Grb2 proteins in human Chang liver and hepatocarcinoma HepG2 cells. Sos1 and p80 proteins dominantly bind to Grb2 fusion proteins in Chang liver, whereas pl00 remarkably associate with Grb2 in HepG2 cells. Also GST-Grb2 SH2 proteins exclusively bound to the p46Shc, p52Shc, and p66Shc are important adaptors of the Ras pathway in HepG2 cells. The pl00 protein has been identified as dynamin II. We observed that the N-SH3 and C-SH3 domains of Grb2 fusion proteins coprecipitated with dynamin II besides Sos1. These results suggest that dynamin II may be a functional molecule involved in Grb2-mediated signaling pathway on Ras activation for tumor progression and differentiation of hepatocarcinoma cells.-
dc.publisherWiley-
dc.titleGrb2 dominantly associates with dynamin II in human hepatocellular carcinoma HepG2 cells-
dc.title.alternativeGrb2 dominantly associates with dynamin II in human hepatocellular carcinoma HepG2 cells-
dc.typeArticle-
dc.citation.titleJournal of Cellular Biochemistry-
dc.citation.number1-
dc.citation.endPage155-
dc.citation.startPage150-
dc.citation.volume84-
dc.contributor.affiliatedAuthorSun Young Yoon-
dc.contributor.affiliatedAuthorMoon Jin Jeong-
dc.contributor.affiliatedAuthorJi Yun Yoo-
dc.contributor.affiliatedAuthorKyung Im Lee-
dc.contributor.affiliatedAuthorByoung-Mog Kwon-
dc.contributor.affiliatedAuthorMi Young Han-
dc.contributor.alternativeName윤선영-
dc.contributor.alternativeName정문진-
dc.contributor.alternativeName유지윤-
dc.contributor.alternativeName이경임-
dc.contributor.alternativeName권병목-
dc.contributor.alternativeName임도선-
dc.contributor.alternativeName이충은-
dc.contributor.alternativeName박영미-
dc.contributor.alternativeName한미영-
dc.identifier.bibliographicCitationJournal of Cellular Biochemistry, vol. 84, no. 1, pp. 150-155-
dc.identifier.doi10.1002/jcb.1275-
dc.subject.keywordDynamin II-
dc.subject.keywordGrb2/SH3 domain-binding proteins-
dc.subject.keywordHepatocarcinoma HepG2 cell-
dc.subject.localDynamin II-
dc.subject.localdynamin II-
dc.subject.localGrb2/SH3 domain-binding proteins-
dc.subject.localHepatocarcinoma HepG2 cell-
dc.description.journalClassY-
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