Internalization of tenecin 3 by a fungal cellular process is essential for its fungicidal effect on Candida albicans

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dc.contributor.authorDae Hee Kim-
dc.contributor.authorDong Gun Lee-
dc.contributor.authorKil Lyong Kim-
dc.contributor.authorYoung Hoon Lee-
dc.date.accessioned2017-04-19T08:58:42Z-
dc.date.available2017-04-19T08:58:42Z-
dc.date.issued2001-
dc.identifier.issn0014-2956-
dc.identifier.uri10.1046/j.1432-1327.2001.02364.xko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5704-
dc.description.abstractTenecin 3 is a glycine-rich, antifungal protein of 78 residues isolated from the insect Tenebrio molitor larva. As an initial step towards understanding the antifungal mechanism of tenecin 3, we examined how this protein interacts with the pathogenic fungus Candida albicans to exert its antifungal action. Tenecin 3 did not induce the release of a fluorescent dye trapped in the artificial membrane vesicles and it did not perturb the membrane potential of C. albicans by the initial interaction. Fluorescence confocal microscopy and flow cytometric analysis revealed that tenecin 3 is rapidly internalized into the cytoplasmic space in energy-dependent and temperature-dependent manners. This internalization is also dependent on the ionic environment and cellular metabolic states. These results suggest that the internalization of tenecin 3 into the cytoplasm of C. albicans is mediated by a fungal cellular process. The internalized tenecin 3 is dispersed in the cytoplasm, and the loss of cell viability occurs after this internalization.-
dc.publisherWiley-
dc.titleInternalization of tenecin 3 by a fungal cellular process is essential for its fungicidal effect on Candida albicans-
dc.title.alternativeInternalization of tenecin 3 by a fungal cellular process is essential for its fungicidal effect on Candida albicans-
dc.typeArticle-
dc.citation.titleEuropean Journal of Biochemistry-
dc.citation.number16-
dc.citation.endPage4458-
dc.citation.startPage4449-
dc.citation.volume268-
dc.contributor.affiliatedAuthorDong Gun Lee-
dc.contributor.affiliatedAuthorKil Lyong Kim-
dc.contributor.alternativeName김대희-
dc.contributor.alternativeName이동건-
dc.contributor.alternativeName김길룡-
dc.contributor.alternativeName이영훈-
dc.identifier.bibliographicCitationEuropean Journal of Biochemistry, vol. 268, no. 16, pp. 4449-4458-
dc.identifier.doi10.1046/j.1432-1327.2001.02364.x-
dc.subject.keywordAntifungal protein-
dc.subject.keywordFungus-
dc.subject.keywordInsect-
dc.subject.keywordInternalization-
dc.subject.keywordTenecin 3-
dc.subject.localAntifungal protein-
dc.subject.localFungus-
dc.subject.localfungus-
dc.subject.localinsect-
dc.subject.localinsects-
dc.subject.localInsect-
dc.subject.localInternalization-
dc.subject.localTenecin 3-
dc.description.journalClassY-
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