Optimization of uracil addition for curdlan (β-1 -> 3-glucan) production by Agrobacterium sp.

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Title
Optimization of uracil addition for curdlan (β-1 -> 3-glucan) production by Agrobacterium sp.
Author(s)
Jung Heon Lee; In Young Lee
Bibliographic Citation
Biotechnology Letters, vol. 23, no. 14, pp. 1131-1134
Publication Year
2001
Abstract
Uracil, acting as a precursor of UDP-glucose, served as an activator on the production of curdlan with Agrobacterium sp. (ATCC 31750). The time of adding uracil was important to improve curdlan production. When uracil was added after ammonium depletion (at 26 h), it was used as a nitrogen source for cell growth. Although the cell concentration increased, the curdlan production was decreased. If uracil was added at 46 h, then uracil was degraded slowly but still activated curdlan production. With the addition of both sucrose (200 g) and uracil (1.5 g), the curdlan production was increased up to 93 g 1-1 after 160 h fermentation.
Keyword
AgrobacteriumCurdlanUracil
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1010516001444
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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