Molecular cloning and characterization of Mycobacterium bovis BCG pcp gene encoding pyrrolidone carboxyl peptidase

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Title
Molecular cloning and characterization of Mycobacterium bovis BCG pcp gene encoding pyrrolidone carboxyl peptidase
Author(s)
Jin Koo Kim; Se Jin Kim; Hee Gu Lee; Jong-Seok Lim; Sang Jae Kim; Sang Hyun Cho; Won Hwa Jeong; In Seong Choe; Tai Wha Chung; Sang Gi Paik; Yong Kyung Choe
Bibliographic Citation
Molecules and Cells, vol. 12, no. 3, pp. 347-352
Publication Year
2001
Abstract
The Mycobacterium bovis bacilli Calmette-Guerin (BCG) pcp gene that encodes the pyrrolidone carboxyl peptidase (Pcp) was cloned from a λgt11 genomic library and sequenced. The nucleotide sequence contains a 669 bp open reading frame coding for a protein of 222 amino acid residues with a calculated molecular mass of 23,209 Da. The deduced amino acid sequence is highly homologous to the Pcps from Bacillus amyloliquefaciens, Pseudomonas fluorescens, Bacillus subtilis, Streptococcus pyogenes, and Staphylococcus aureus. A multiple sequence alignment revealed highly conserved domains. The BCG pcp gene was overexpressed in Escherichia coli. The Pcp was purified to homogeneity. The recombinant protein was further confirmed by an enzymatic assay.
Keyword
pcp genepyrrolidone carboxyl peptidase (Pcp)mycobacterium bovis BCG
ISSN
1016-8478
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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