Estimation of distribution of a commensal thermophile in soil by competitive quantitative PCR and terminal restriction fragment length polymorphism analysis

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Title
Estimation of distribution of a commensal thermophile in soil by competitive quantitative PCR and terminal restriction fragment length polymorphism analysis
Author(s)
Sung-Keun Rhee; Seung Pyo Hong; Jin-Woo Bae; Che Ok Jeon; Seung Goo LeeJae Jun SongHa Ryoung Poo; Moon Hee Sung
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 11, no. 6, pp. 940-945
Publication Year
2001
Abstract
Symbiobacterium toebii has been previously reported as a novel commensal thermophile exhibiting a commensal interaction with thermophilic Geobacillus sp. SK-1. We investigated the distribution of this commensal thermophile in various soils using molecular methods, such as quantitative PCR and terminal restriction fragment polymorphism analysis. Based on a nested competitive quantitative PCR, the 16S rDNA of the commensal thermophile was only detected in compost soils at about 1.0×104 copies per gram of soil, corresponding to 0.25×104 cells per gram of soil. However, in an enrichment experiment at 60°C, about 1.0×108 copies of 16S rDNA molecules were detected per ml of enriched culture broth for all the soils, and more than 0.1 mM indole accumulated as the product of commensal bacterial growth. When incubated at 30°C, neither the 16S rDNA of the commensal bacterium nor any indole accumulation was detected. Accordingly, even though the 16S rDNA of the bacterium was only detected in the compost soils by a nested PCR, the presence of the 16S rDNA molecules of commensal thermophile and accumulation of indole in all the enriched cultures appeared to indicate that the commensal thermophile is widely distributed in various soils.
Keyword
commensaldistributionsoilsymbiobacteriumthermophile
ISSN
1017-7825
Publisher
South Korea
Type
Article
Appears in Collections:
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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