Removal of 5 '- and 3 '-untranslated regions enhances erythropoietin gene expression in mammalian cells

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Title
Removal of 5 '- and 3 '-untranslated regions enhances erythropoietin gene expression in mammalian cells
Author(s)
J H Park; C Kim; W B Kim; Young Kook Kim; S Y Lee; J M Yang
Bibliographic Citation
Molecular Biology, vol. 35, no. 3, pp. 350-353
Publication Year
2001
Abstract
The effect of the 5′-and 3′-untranslated regions (UTR) of human erythropoietin gene (gEpo) on its expression in mammalian cells was investigated. 5′-UTR-deleted, 3′-UTR-deleted, and 5′,3′-UTR-deleted gEpos were transfected into COS-7 cells, and the amount of transiently expressed Epo was measured. Cells transfected with the 5′,3′-UTR-deleted gEpo showed the highest expression, 2.6 times that with wild-type gEpo. Northern blot analysis indicated that the enhanced expression of 5′,3′-UTR-deleted gEpo might be related with the transcription efficiency.
Keyword
ErythropoietinSRα promoterTranscription regulationUntranslated region
ISSN
0026-8933
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1010466526838
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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