Identification of total extracellular fibrinase from Bacillus sp. DJ using one- or two- dimensional fibrin Zymography for proteomic approach

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dc.contributor.authorNack Shick Choi-
dc.contributor.authorJin Young Lee-
dc.contributor.authorKab Seog Yoon-
dc.contributor.authorKyoung Yoen Han-
dc.contributor.authorSeung Ho Kim-
dc.date.accessioned2017-04-19T08:58:46Z-
dc.date.available2017-04-19T08:58:46Z-
dc.date.issued2001-
dc.identifier.issn1017-7825-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5729-
dc.description.abstractAn extracellular fibrinolytic-enzyme-producing bacterium was isolated from Doen-Jang, a Korean traditional fermented food, and identified as Bacillus sp. DJ based on its morphology and cellular fatty acid composition. The total extracellular fibrinase (EF) from Bacillus sp. DJ was analyzed using three fibrin zymographic techniques, SDS-fibrin zymography (SDS-FZ), isoelectrofocucing-fibrin zymography (IEF-FZ), and a two-dimensional SDS-fibrin zymographic analysis (2D SDS-FZ). As a result, the EF map of Bacillus sp. DJ was established. The results suggest that the 2D SDS-FZ method will be a useful tool for the proteomic approach for many other bacterial proteases.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleIdentification of total extracellular fibrinase from Bacillus sp. DJ using one- or two- dimensional fibrin Zymography for proteomic approach-
dc.title.alternativeIdentification of total extracellular fibrinase from Bacillus sp. DJ using one- or two- dimensional fibrin Zymography for proteomic approach-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number6-
dc.citation.endPage1114-
dc.citation.startPage1111-
dc.citation.volume11-
dc.contributor.affiliatedAuthorNack Shick Choi-
dc.contributor.affiliatedAuthorJin Young Lee-
dc.contributor.affiliatedAuthorKab Seog Yoon-
dc.contributor.affiliatedAuthorKyoung Yoen Han-
dc.contributor.affiliatedAuthorSeung Ho Kim-
dc.contributor.alternativeName최낙식-
dc.contributor.alternativeName이진영-
dc.contributor.alternativeName윤갑석-
dc.contributor.alternativeName한경연-
dc.contributor.alternativeName김승호-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 11, no. 6, pp. 1111-1114-
dc.subject.keywordbacillus-
dc.subject.keywordfibrinase-
dc.subject.keywordproteomics-
dc.subject.keywordtwo-dimensional fibrin zymography-
dc.subject.localBacillus-
dc.subject.localbacillus-
dc.subject.localfibrinase-
dc.subject.localproteomics-
dc.subject.localtwo-dimensional fibrin zymography-
dc.description.journalClassY-
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