Production of oleamide, a functional lipid, by Streptomyces sp. KK90378

Cited 0 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorHo Jeong Kwon-
dc.contributor.authorSung Eun Hwang-
dc.contributor.authorJae Taek Han-
dc.contributor.authorChang Jin Kim-
dc.contributor.authorJung Rae Rho-
dc.contributor.authorJong Heon Shin-
dc.date.accessioned2017-04-19T08:58:50Z-
dc.date.available2017-04-19T08:58:50Z-
dc.date.issued2001-
dc.identifier.issn1017-7825-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5748-
dc.description.abstractOleamide (cis-9-octadecenamide) is an endogenous primary amide of fatty acid that is produced in small amounts in animal brains. It is known to induce sleep and to lower temperature by destroying the lipid plasma membrane structure of cells, thereby disclosing gap junction channels. To develop a new biological production method for oleamide, a screening program was conducted to isolate a microorganism producing oleamide. Among 1,500 soil microorganisms tested, KK90378 exhibited a potent positive reaction with Dragendorff's reagent, used to detect the primary amide of oleamide. KK90378 was identified as a Streptomyces species based on cultural and morphological characteristics, the presence of diaminopimelic acid in the cell wall, and the sugar patterns for the whole-cell extract. Streptomyces sp. KK90378 produced oleamide 3 days after culture at 28°C, pH 7.2. A series of purification steps, including hexane extraction, silica gel column, and preparative thin layer chromatographies, were performed for the purification of oleamide. A spectrophotometric analysis using 1H, 13C-NMR, and GC-MS confirmed that the chemical structure of the purified oleamide was identical to that of authentic oleamide.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleProduction of oleamide, a functional lipid, by Streptomyces sp. KK90378-
dc.title.alternativeProduction of oleamide, a functional lipid, by Streptomyces sp. KK90378-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number6-
dc.citation.endPage1023-
dc.citation.startPage1018-
dc.citation.volume11-
dc.contributor.affiliatedAuthorChang Jin Kim-
dc.contributor.alternativeName권호정-
dc.contributor.alternativeName황승은-
dc.contributor.alternativeName한재택-
dc.contributor.alternativeName김창진-
dc.contributor.alternativeName노정래-
dc.contributor.alternativeName신종헌-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 11, no. 6, pp. 1018-1023-
dc.subject.keywordmicrobial production-
dc.subject.keywordoleamide-
dc.subject.keywordsleep inducing agent-
dc.subject.keywordstreptomyces sp.-
dc.subject.localmicrobial production-
dc.subject.localMicrobial production-
dc.subject.localoleamide-
dc.subject.localsleep inducing agent-
dc.subject.localStreptomyces sp-
dc.subject.localStreptomyces sp.-
dc.subject.localstreptomyces sp.-
dc.description.journalClassY-
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.