Complestatin antagonizes the AMPA/Kainate-induced neurotoxicity in cultured chick telencephalic neurons

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Title
Complestatin antagonizes the AMPA/Kainate-induced neurotoxicity in cultured chick telencephalic neurons
Author(s)
Ick Dong Yoo; Bong Sik Yun; In Ja Ryoo; Soo Young Lee; Myeong Heon Shin; Sei Kwan Oh
Bibliographic Citation
Neurochemical Research, vol. 27, no. 4, pp. 337-343
Publication Year
2002
Abstract
Excitatory amino acids are known to induce considerable neurotoxicity in central nervous system. In the present study, the neurotoxicity was induced by application of kainate or AMPA in chick telencephalic neuron, and neuroprotective activity was tested with complestatin that was isolated from streptomyces species. In cultured telencephalic neurons exposed to 500 μM kainate for 2 days, the AMPA/kainate receptor antagonist 6,7-dinitroquinoxaline-2,3-dione (DNQX, 5 μM) completely blocked kainate-induced neurotoxicity. Also, complestatin (0.5 μM) completely blocked kainate-induced neuronal injury at a concentration lower than that required for prototype AMPA/kainate receptor antagonist DNQX. In addition, complestatin blocked AMPA-induced neurotoxicity when the neurons were pretreated with cyclothiazide, a desensitization blocker of AMPA receptor. Surprisingly, when the onset of the treatment was delayed for 6 hours, complestatin led to a reduction in kainate-induced neuronal injury. While inhibition of protein kinase C (PKC) by staurosporin induced neurotoxicity, that was blocked by complestatin. Activation of PKC by phorbol dibutyrate partially inhibited the kainate-induced neurotoxicity. These results suggest that complestatin may be used as an anti-excitotoxic agent and involved in the PKC activation contributing to inhibition of neurotoxicity.
Keyword
AMPA/kainiteChick neuronComplestatinNeurotoxicity
ISSN
0364-3190
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1014919531306
Type
Article
Appears in Collections:
Ochang Branch Institute > Anticancer Agent Research Center > 1. Journal Articles
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