Characterization of Symbiobacterium toebii, an obligate commensal thermophile isolated from compost

Cited 30 time in scopus
Metadata Downloads
Title
Characterization of Symbiobacterium toebii, an obligate commensal thermophile isolated from compost
Author(s)
Sung-Keun Rhee; Che Ok Jeon; Jin-Woo Bae; Kwang Kim; Jae Jun Song; Joong Jae Kim; Seung Goo Lee; Hong Ik Kim; Seung Pyo Hong; Yoon Ho Choi; Su Mi Kim; Moon Hee Sung
Bibliographic Citation
Extremophiles, vol. 6, no. 1, pp. 57-64
Publication Year
2002
Abstract
A symbiotic thermophilic bacterium, strain SC-1, was isolated from hay compost (toebi) in Korea. The new isolate exhibited an obligate commensal interaction with a thermophilic Geobacillus strain and required crude extracts and/or culture supernatant from Geobacillus sp. SK-1 for axenic growth. The growth factors from Geobacillus sp. SK-1 were irreversibly inactivated by phenol or protease treatment, suggesting that they might be proteins. The cells of strain SC-1 were non-spore forming, nonmotile rods that were stained Gram-negatively. The isolate was a microaerophilic heterotroph. Growth was observed between 45° and 70°C (optimum: 60°C; 2.4-h doubling time) and pH 6.0 and 9.0 (optimum: pH 7.5). The G+C content of the genomic DNA was 65 mol%, and the major quinones were MK-6 and MK-7. A phylogenetic analysis of its 16S rDNA sequence indicated that strain SC-1 is closely related to Symbiobacterium thermophilum and so was named Symbiobacterium toebii on the basis of its physiological and molecular properties.
Keyword
CommensalismCompostMicroaerophilicSymbiobacterium toebiiSymbiotic bacteriumThermophile
ISSN
1431-0651
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s007920100233
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
Division of Biomaterials Research > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.