Activation of dynamin I gene expression by Sp1 and Sp3 is required for neuronal differentiation of N1E-115 cells

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Title
Activation of dynamin I gene expression by Sp1 and Sp3 is required for neuronal differentiation of N1E-115 cells
Author(s)
Ji Yun Yoo; Moon Jin Jeong; Byoung-Mog Kwon; Man Wook Hur; Young Mee Park; Mi Young Han
Bibliographic Citation
Journal of Biological Chemistry, vol. 277, no. 14, pp. 11904-11909
Publication Year
2002
Abstract
Dynamin I is a key molecule required for the recycling of synaptic vesicles in neurons, and it has been known that dynamin I gene expression is induced during neuronal differentiation. Our previous studies established that neuronal restriction of dynamin I gene expression is controlled by Spl and nuclear factor-KB-like element-1. Here, using a series of deletion constructs and site-directed mutation, we found that transcription of dynamin I gene during neuronal differentiation of N1E-115 cells is controlled primarily by the Spl element located between -13 to -4 bp of the dynamin I promoter. Gel shift analysis demonstrated that in addition to SpI, Sp3 could interact with this Spl element. The requirement for Sp family transcription factors in dynamin I gene expression was confirmed by using mithramycin, an inhibitor of Sp1/Sp3 binding. Mithramycin repressed dynamin I gene expression and resulted in blocking of neuronal differentiation of NIE-115 cells. The localization of the dynamin I protein was also restricted in the peripheral region of the nucleus by the mithramycin treatment. Thus, all of our results suggest that induction of dynamin I gene expression during N1E-115 cell differentiation is modulated by Sp1/Sp3 interactions with the dynamin I promoter, and its expression is important for neuronal differentiation of the N1E-115 cells.
ISSN
0021-9258
Publisher
Amer Soc Biochemistry Molecular Biology Inc
DOI
http://dx.doi.org/10.1074/jbc.M111788200
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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