The western red cedar (Thuja plicata) 8-8′DIRIGENT family displays diverse expression patterns and conserved monolignol coupling specificity

Cited 70 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorMyoung K Kim-
dc.contributor.authorJae Heung Jeon-
dc.contributor.authorMasayuki Fujita-
dc.contributor.authorLaurence B Davin-
dc.contributor.authorNorman G Lewis-
dc.date.accessioned2017-04-19T08:58:55Z-
dc.date.available2017-04-19T08:58:55Z-
dc.date.issued2002-
dc.identifier.issn0167-4412-
dc.identifier.uri10.1023/A:1014940930703ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5786-
dc.description.abstractThe isolation and characterization of a multigene family of the first class of dirigent proteins (namely that mainly involved in 8-8′ coupling leading to (+)-pinoresinol in this case) is reported, this comprising of nine western red cedar (Thuja plicata) DIRIGENT genes (DIR1-9) of 72-99.5% identity to each other. Their corresponding cDNA clones had coding regions for 180-183 amino acids with each having a predicted molecular mass of ca 20 kDa including the signal peptide. Real time-PCR established that the DIRIGENT isovariants were differentially expressed during growth and development of T. plicata (P < 0.05). The phylogenetic relationships and the rates and patterns of nucleotide substitution suggest that the DIRIGENT gene may have evolved via paralogous expansion at an early stage of vascular plant diversification. Thereafter, western red cedar paralogues have maintained an high homogeneity presumably via a concerted evolutionary mode. This, in turn, is assumed to be the driving force for the differential formation of 8-8′-linked pinoresinol derived (poly)lignans in the needles, stems, bark and branches, as well as for massive accumulation of 8-8′-linked plicatic acid-derived (poly)lignans in heartwood.-
dc.publisherSpringer-
dc.titleThe western red cedar (Thuja plicata) 8-8′DIRIGENT family displays diverse expression patterns and conserved monolignol coupling specificity-
dc.title.alternativeThe western red cedar (Thuja plicata) 8-8′DIRIGENT family displays diverse expression patterns and conserved monolignol coupling specificity-
dc.typeArticle-
dc.citation.titlePlant Molecular Biology-
dc.citation.number2-
dc.citation.endPage214-
dc.citation.startPage199-
dc.citation.volume49-
dc.contributor.affiliatedAuthorJae Heung Jeon-
dc.contributor.alternativeName김명-
dc.contributor.alternativeName전재흥-
dc.contributor.alternativeNameFujita-
dc.contributor.alternativeNameDavin-
dc.contributor.alternativeNameLewis-
dc.identifier.bibliographicCitationPlant Molecular Biology, vol. 49, no. 2, pp. 199-214-
dc.identifier.doi10.1023/A:1014940930703-
dc.subject.keyword8-8′-
dc.subject.keywordDIRIGENT multigene family-
dc.subject.keywordlignans and lignins-
dc.subject.keywordreal-time PCR-
dc.subject.keywordrecombinant protein-
dc.subject.keywordstereoselective phenoxy radical coupling-
dc.subject.keywordwestern red cedar (Thuja plicata)-
dc.subject.local8-8′-
dc.subject.localDIRIGENT multigene family-
dc.subject.locallignans and lignins-
dc.subject.localReal-time PCR-
dc.subject.localreal time PCR-
dc.subject.localReal time PCR-
dc.subject.localreal-time PCR-
dc.subject.localRecombinant Protein-
dc.subject.localrecombinant protein-
dc.subject.localrecombinant proteins-
dc.subject.localRecombinant protein-
dc.subject.localecombinant proteins-
dc.subject.localRecombinant proteins-
dc.subject.localstereoselective phenoxy radical coupling-
dc.subject.localwestern red cedar (Thuja plicata)-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.