Relationship between acrylamide concentration and enzymatic activity in an improved single fibrin zymogram gel system

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dc.contributor.authorNack Shick Choi-
dc.contributor.authorByoung Young Kim-
dc.contributor.authorJin Young Lee-
dc.contributor.authorKab Seog Yoon-
dc.contributor.authorKyoung Yoen Han-
dc.contributor.authorSeung Ho Kim-
dc.date.accessioned2017-04-19T08:58:56Z-
dc.date.available2017-04-19T08:58:56Z-
dc.date.issued2002-
dc.identifier.issn1225-8687-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5788-
dc.description.abstractBased on the zymography analysis, Bacillus sp. DJ-4 (screened from Doen-Jang, a Korean traditional fermented food) secretes seven extracellular fibrinolytic enzymes (EFEs; 68, 64, 55, 45, 33, 27, and 13 kDa) in culture broth. These seven EFEs were analyzed by newly applied SDS-fibrin zymography combined with gradient polyacrylamide (SDS-FZGP). This improved gel system was used with a 5-20% acrylamide gradient in a fibrin zymogram gel for the separation of proteins with molecular masses from below 10 kDa to over 100 kDa on one gel plate. Using this system, high molecular weight bands (HMWBs) were clearly and sharply resolved. We also examined the relationship between an acrylamide concentration and the enzymatic activity of EFE using densitometric analysis.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleRelationship between acrylamide concentration and enzymatic activity in an improved single fibrin zymogram gel system-
dc.title.alternativeRelationship between acrylamide concentration and enzymatic activity in an improved single fibrin zymogram gel system-
dc.typeArticle-
dc.citation.titleBMB Reports-
dc.citation.number2-
dc.citation.endPage238-
dc.citation.startPage236-
dc.citation.volume35-
dc.contributor.affiliatedAuthorNack Shick Choi-
dc.contributor.affiliatedAuthorJin Young Lee-
dc.contributor.affiliatedAuthorKab Seog Yoon-
dc.contributor.affiliatedAuthorKyoung Yoen Han-
dc.contributor.affiliatedAuthorSeung Ho Kim-
dc.contributor.alternativeName최낙식-
dc.contributor.alternativeName김병영-
dc.contributor.alternativeName이진영-
dc.contributor.alternativeName윤갑석-
dc.contributor.alternativeName한경연-
dc.contributor.alternativeName김승호-
dc.identifier.bibliographicCitationBMB Reports, vol. 35, no. 2, pp. 236-238-
dc.identifier.doi10.5483/bmbrep.2002.35.2.236-
dc.subject.keywordbacillus-
dc.subject.keywordextracellular protease-
dc.subject.keywordfibrin zymography-
dc.subject.localBacillus-
dc.subject.localbacillus-
dc.subject.localextracellular protease-
dc.subject.localFibrin zymography-
dc.subject.localfibrin zymography-
dc.description.journalClassY-
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