Studies on microbial ecology of actinomycetes in tideland soils = 서해 아암도 갯벌토양 미생물의 개체군 분석 및 RAPD 분석에 의한 방선균의 생태학적 연구

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Title
Studies on microbial ecology of actinomycetes in tideland soils = 서해 아암도 갯벌토양 미생물의 개체군 분석 및 RAPD 분석에 의한 방선균의 생태학적 연구
Author(s)
Young Ju Cho; Jeong Hwan Kim; Eun Soo Jeon; Sang Mi Lee; Dong Jin park; Jae Chan Lee; hyang Burm Lee; Chang Jin Kim
Bibliographic Citation
Korean Journal of (Applied) Microbiology & Biotechnology, vol. 30, no. 1, pp. 79-85
Publication Year
2002
Abstract
Ecological characteristics of microorganisms in tideland soils were studied by investigation of microbial diversity and population. Twenty soil samples were taken at surface, 10, 20 and 30 cm depth each. Bacteria, actinomycetes and fungi were isolated on each selective isolation medium containing different concentration of NaC1. Actinomycetes were the most isolated from soil samples taken at 10 cm depth and isolated by humic acid-vitamin (HV) medium without sea water or salt. Twenty nine strains of actinomycetes were isolated at surface soil and 74, 39, 37 strains were at 10, 20, and 30 cm depth, respectively. All these isolates were analysed and grouped by random amplified polymorphic DNA (RAPD)-PCR analysis. Many of the isolates were clustered into Microtetraspora and Pseudonocardia. Fungal isolates were highly distributed at the surface soil and isolated well on potato dextrose agar (PDA) medium with sea water. Bacterial isolates were higly distributed at surface soil and isolated well by nutrient medium without sea water or salt. Soil samples taken at 10 cm depth showed the highest microbial diversity and population.
Keyword
actinomycetesmicrobial diversityRAPDtidelandUPGMA
ISSN
0257-2389
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
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