Transgenic tomato plants that overexpress superoxide dismutase in fruits = 토마토 과실에서 superoxide dismutase를 고발현하는 형질전환 식물체

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Title
Transgenic tomato plants that overexpress superoxide dismutase in fruits = 토마토 과실에서 superoxide dismutase를 고발현하는 형질전환 식물체
Author(s)
Eun Jeong Park; Heang Soon Lee; Suk Yoon Kwon; Kwan Sam Choi; Sang Soo Kwak
Bibliographic Citation
Korean Journal of Plant Biotechnology (Tissue Culture), vol. 29, no. 1, pp. 7-13
Publication Year
2002
Abstract
Superoxide dismutase (SOD) plays an important role in cellular defense against oxidative stress in plants. We have developed transgenic tomato plants overexpressing a cassava SOD in fruits. Three transgenic tomato plants (one from cv. Pink forcer and two from cv. Koko) using a new vector system, ASOp :: . mSOD1/pBI101, harboring ascorbate oxidase promoter (ASOp) expressing dominantly in cucumber fruits, CuZnSOD cDNA (mSOD1) isolated from cultured cells of cassava, and nptll gene as a selectable marker were successfully developed. SOD specific activity (units/mg protein) in transgenic fruits of both cultivars was increased with maturation of the fruits. SOD specific activity of well-mature fruits in transgenic Pink forcer and Koko showed approximately 1.6 and 2.2 times higher than control fruits, respectively. The strength of SOD isoenzyme bands well reflected the SOD activity during the fruit maturation. These results suggested that SOD gene was properly introduced into tomato fruits in a fruit-dominant expression manner by ASO promoter.
Keyword
ascorbate oxidase promoterlycopersiconesculentumoxidative stressplant bioreactorsuperoxide
ISSN
1015-5880
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.5010/JPB.2002.29.1.007
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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