Overexpression of urokinase-type plasminogen activator in human gastric cancer cell line (AGS) induces tumorigenicity in severe combined immunodeficient mice

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dc.contributor.authorYang Kyu Choi-
dc.contributor.authorByung Il Yoon-
dc.contributor.authorYoon Hoh Kook-
dc.contributor.authorYoung Suk Won-
dc.contributor.authorJin Hyun Kim-
dc.contributor.authorChul Ho Lee-
dc.contributor.authorByung Hwa Hyun-
dc.contributor.authorGoo Taeg Oh-
dc.contributor.authorJohn Sipley-
dc.contributor.authorDae Yong Kim-
dc.date.accessioned2017-04-19T08:59:01Z-
dc.date.available2017-04-19T08:59:01Z-
dc.date.issued2002-
dc.identifier.issn0910-5050-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5823-
dc.description.abstractThe significance of urokinase-type plasminogen activator (uPA) expression in gastric cancer development was tested by using a human uPA cDNA transfection approach and an in vivo severe combined immunodeficient (SCID) mouse model. The AGS gastric cancer cell line, which has urokinase-type plasminogen-activator receptor (uPAR) but lacks uPA, was transfected with a plasmid containing human uPA cDNA and injected into the backs of SCID mice. Compared with the parent AGS cells, uPA protein secretion in AGS-2-, AGS-4-, and AGS-8-transfected cells increased by 26.1-, 34.6-, and 4.8-fold, respectively (P<0.05). mRNA expression levels of uPA in the AGS-4 clone were much stronger than those in AGS-2 and AGS-8 clones. After the cancer cells (2×106) were injected s.c. into the SCID mice, a palpable mass was observed at the injection site at around 140 days post-injection, followed by accelerated growth of the xenograft up to 180 days post-injection only in the high uPA-producing clone (AGS-4). These results suggest that continuous and high production of uPA by tumor cells is one of the important factors reflecting the malignancy of gastric cancer cells.-
dc.titleOverexpression of urokinase-type plasminogen activator in human gastric cancer cell line (AGS) induces tumorigenicity in severe combined immunodeficient mice-
dc.title.alternativeOverexpression of urokinase-type plasminogen activator in human gastric cancer cell line (AGS) induces tumorigenicity in severe combined immunodeficient mice-
dc.typeArticle-
dc.citation.titleJapanese Journal of Cancer Research-
dc.citation.number2-
dc.citation.endPage156-
dc.citation.startPage151-
dc.citation.volume93-
dc.contributor.affiliatedAuthorYang Kyu Choi-
dc.contributor.affiliatedAuthorYoung Suk Won-
dc.contributor.affiliatedAuthorChul Ho Lee-
dc.contributor.affiliatedAuthorByung Hwa Hyun-
dc.contributor.affiliatedAuthorGoo Taeg Oh-
dc.contributor.alternativeName최양규-
dc.contributor.alternativeName윤병일-
dc.contributor.alternativeName국윤호-
dc.contributor.alternativeName원영석-
dc.contributor.alternativeName김진현-
dc.contributor.alternativeName이철호-
dc.contributor.alternativeName현병화-
dc.contributor.alternativeName오구택-
dc.contributor.alternativeNameSipley-
dc.contributor.alternativeName김대용-
dc.identifier.bibliographicCitationJapanese Journal of Cancer Research, vol. 93, no. 2, pp. 151-156-
dc.identifier.doi10.1111/j.1349-7006.2002.tb01253.x-
dc.subject.keywordAGS-
dc.subject.keywordgastric cancer-
dc.subject.keywordSCID-
dc.subject.keywordtransfection-
dc.subject.keyworduPA-
dc.subject.localAGS-
dc.subject.localgastric cancer-
dc.subject.localGastric cancer (GC)-
dc.subject.localGastric cancer-
dc.subject.localSCID-
dc.subject.localTransfection-
dc.subject.localtransfection-
dc.subject.localUPA-
dc.subject.localuPA-
dc.description.journalClassN-
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > Laboratory Animal Resource & Research Center > 1. Journal Articles
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