Molecular cloning and phylogenetic analysis of new human endogenous retrovirus HERV-W family in cancer cells

Cited 11 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorJoo Mi Yi-
dc.contributor.authorHwan Mook Kim-
dc.contributor.authorWon Ho Lee-
dc.contributor.authorHeui Soo Kim-
dc.date.accessioned2017-04-19T08:59:02Z-
dc.date.available2017-04-19T08:59:02Z-
dc.date.issued2002-
dc.identifier.issn03438651-
dc.identifier.uri10.1007/s00284-001-0033-5ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5834-
dc.description.abstractA human endogenous retroviral family (HERV-W) has recently been described that is related to multiple sclerosis-associated retrovirus (MSRV) sequences. By using the PCR approach with human genomic DNA derived from cancer cell lines (HepG2, Jurkat, MCF7, UO-31), five env fragments of HERV-W family were newly identified and analyzed. They showed a high degree of nucleotide sequence similarity (94-99%) with that of the HERV-W. Translation of the env fragments showed no frameshift and termination codon by deletion/insertion or point mutation in clones HepG2-1 and JUR-3. The ratio of synonymous to non-synonymous substitutions indicated that negative selective pressure is acting on HepG2-1 and JUR-3 sequences. These env gene sequences could be associated with an active provirus in human cancer cells (HepG2 and Jurkat). The HepG2-1 and JUR-3 showed sister relationship with the HERV-W and W-7-1 derived from human Chromosome (Chr) 7. Phylogenetic analysis from the HERV-W family indicated close relationships of the env gene sequences across human chromosomes.-
dc.publisherSpringer-
dc.titleMolecular cloning and phylogenetic analysis of new human endogenous retrovirus HERV-W family in cancer cells-
dc.title.alternativeMolecular cloning and phylogenetic analysis of new human endogenous retrovirus HERV-W family in cancer cells-
dc.typeArticle-
dc.citation.titleCurrent Microbiology-
dc.citation.number3-
dc.citation.endPage220-
dc.citation.startPage216-
dc.citation.volume44-
dc.contributor.alternativeName이주미-
dc.contributor.alternativeName김환묵-
dc.contributor.alternativeName이원호-
dc.contributor.alternativeName김희수-
dc.identifier.bibliographicCitationCurrent Microbiology, vol. 44, no. 3, pp. 216-220-
dc.identifier.doi10.1007/s00284-001-0033-5-
dc.description.journalClassY-
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.