Identification of new endogenous retroviral sequences belonging to the HERV-W family in human cancer cells

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Title
Identification of new endogenous retroviral sequences belonging to the HERV-W family in human cancer cells
Author(s)
Joo Mi Yi; Won Ho Lee; Hwan Mook Kim; Heui Soo Kim
Bibliographic Citation
Intervirology, vol. 44, no. 6, pp. 333-338
Publication Year
2001
Abstract
A human endogenous retroviral family (HERV-W) has recently been identified on chromosome 7 that contains a single complete open reading frame putatively encoding an envelope protein. We have identified fifteen HERV-W families on human genomic DNA using a monochromosomal panel in our previous study. In order to identify additional families, we examined genomic DNA derived from cancer cell lines (A549, AZ521, OVCAR3, RT4) using the PCR approach. Five env fragments of a HERV-W family were newly identified and analyzed. They showed a high degree of nucleotide sequence similarity (93-97%) to that of the other HERV-W families. Translation of the env fragments showed no frameshift and termination codon by deletion/insertion or point mutation in clones A549-2, AZ521-3, and OVCAR-3. The ratio of synonymous to nonsynonymous substitutions indicated that negative selective pressure is acting on A549-2, AZ521-3, and OVCAR-3 sequences. These env gene sequences could be associated with an active provirus in human cancer cells (A549, AZ521, OVCAR3).
Keyword
Cancer cellsEndogenous retrovirusHERV-W familyPCRPhylogeny
ISSN
0300-5526
Publisher
Karger
DOI
http://dx.doi.org/10.1159/000050067
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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