Pathogen-induced expression of cyclo-oxygenase homologue in hot pepper (Capsicum annuum cv. Pukang)

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Title
Pathogen-induced expression of cyclo-oxygenase homologue in hot pepper (Capsicum annuum cv. Pukang)
Author(s)
Young Cheol Kim; So Young Yi; Hyung Gon Mang; Young Sam Seo; Woo Taek Kim; Do Il Choi
Bibliographic Citation
Journal of Experimental Botany, vol. 53, no. 367, pp. 383-385
Publication Year
2002
Abstract
The hypersensitive reaction (HR) in plants is typified by a rapid and localized cell death at the site of pathogen infection. To understand better the molecular and cellular defence mechanism controlling HR, hot pepper leaves (Capsicum annuum cv. Pukang) were inoculated with the soybean pustule pathogen Xanthomonas campestris pv. glycine 8ra. By using the DD-PCR technique, a cDNA fragment was identified that exhibited a sequence similarity to the recently identified tobacco pathogen-induced oxygenase (PIOX) with homology to animal cyclo-oxygenase (COX). Subsequently, the full-length cDNA clone, pCa-COX1, encoding the COX homologue from the pathogen-inoculated hot pepper leaf cDNA library was isolated. The deduced amino acid sequence of Ca-COX1 shares 85.8% identity with tobacco PIOX and displays a significant degree of sequence identity (21.7-23.7%) with mammalian COXs. The expression of Ca-COX1 was markedly induced at 4-12 h after pathogen infection, while HR cell death on pepper leaves appeared at approximately 15 h post-inoculation. These results are consistent with the notion that the lipid-derived signalling pathway is involved in the initial response of hot pepper plants to pathogen infection.
Keyword
Cyclo-oxygenaseHot pepperHypersensitive reactionPathogen infectionPlant defence mechanism
ISSN
0022-0957
Publisher
Oxford Univ Press
DOI
http://dx.doi.org/10.1093/jexbot/53.367.383
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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