Glycolipid biosurfactants produced by Pseudomonas aeruginosa D2D2 from diesel-contaminated soil

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Title
Glycolipid biosurfactants produced by Pseudomonas aeruginosa D2D2 from diesel-contaminated soil
Author(s)
Hye Joon Moon; Young Kuong Lim; Hee Sik Kim; Dae Young Kwon; Wook Jin Chung
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 12, no. 3, pp. 371-376
Publication Year
2002
Abstract
A biosurfactant-producing bacterial strain was selected from diesel-contaminated soil by measuring the oil-film collapsing activity and identified as Pseudomonas aeruginosa D2D2. When glucose and olive oil were used as carbon sources, 11.46 g/l of biosurfactant was obtained. Based on TLC analysis, the biosurfactant produced from P. aeruginosa D2D2 was identified as a glycolipid, consisting of two types of biosurfactants (Type I and Type II). The purified glycolipid reduced the surface tension of the culture from 72 dyne/cm to 27 dyne/cm. The hydrophilic and hydrophobic moiety of the biosurfactant were rhamnose and β-hydroxydecanoic acid, as determined by FAB-MS and NMR analyses, respectively.
Keyword
biosurfactantglycolipidpseudomonas aeruginosa D2D2rhamnolipiddiesel fuelpseudomonas aeruginosasoil pollution
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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