A new spin filter for high density culture and ethanol production by Saccharomyces cerevisiae

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Title
A new spin filter for high density culture and ethanol production by Saccharomyces cerevisiae
Author(s)
Hyun Soo Moon; Dong Joon Lim; Gu Young Song; Hyun Ah Kang; Seung Wook Kim; Ik Hwan Kim; Suk In Hong
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 12, no. 3, pp. 406-410
Publication Year
2002
Abstract
A new spin filter consisting of 50 μm (nominal pore size) depth filters rolled on a stainless steel grid was developed, using Saccharomyces cerevisiae as a model suspension cell to evaluate the spin filter performance. In a 1.8-1 fermentor with a rotation speed of 300 rpm and perfusion rate of 4 ml/min, a cell concentration of 49 g/l and ethanol concentration of 45 g/l from 100 g/l glucose could be obtained in a perfusion culture. The major mechanisms for cell separation used by the large-pore spin filter appeared to be centrifugal force and pivotal movement of the cells in the spin filter.
Keyword
centrifugal forcehigh density culturepivotal movementspin filteryeast
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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