Identification of Stenotrophomonas maltophilia LK-24 and its degradability of crystal violet

Abstract

A number of soil and wastewater samples were collected from the vicinity of an effluent treatment plant for the chemical industry. Several microorganisms were screened for their ability to decolorize the triphenylmethane group of dyes. As a result, a novel crystal violet dye-degrading strain LK-24 was isolated. Taxonomic identification including 16S rDNA sequencing and phylogenetic analysis indicated that the isolate had a 99.5% homology in its 16S rDNA base sequence with Stenotrophomonas maltophilia. The triphenylmethane dye, crystal violet, was degraded extensively by growing cells of Stenotrophomonas maltophilia LK-24 in agitated liquid cultures, although their growth was strongly inhibited in the initial stage of incubation. This group of dyes is toxic, depending on the concentration used. The dye was significantly degraded at a relatively lower concentration, below 100 μg ml-1, yet the growth of the cells was totally suppressed at a dye concentration of 250 μg ml-1. The degradation products of crystal violet were identified as 4,4′-bis(dimethylamino)-benzophenone and ρ-dimethylaminophenol by Gas chromatography-Mass spectrometry. The 4,4′-bis(dimethylamino)-benzophenone was easily obtained in a reasonable yield, as it was not metabolized further by S. maltophilia LK-24; however, the ρ-dimethylaminophenol was not easily identifiable, as it was further metabolized.