Isolation and biological properties of novel cell cycle inhibitors, HY558, isolated from Penicillium minioluteum F558

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Title
Isolation and biological properties of novel cell cycle inhibitors, HY558, isolated from Penicillium minioluteum F558
Author(s)
Chul Hoon Lee; Hae Young Lim; Min Kyoung Kim; Youl Hee Cho; Deok Kun Oh; Chang Jin Kim; Young Ho Lim
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 12, no. 3, pp. 470-475
Publication Year
2002
Abstract
In the course of screening for a novel cell cycle inhibitor, a potent Cdk 1 inhibitor, HY558, was found from the culture broth of Penicillium minioluteum F558 isolated from a soil sample. The molecular ion of HY558 was identified at m/z 329 (MH+) with a molecular formula of C20H44ON2. HY558 exhibited selective antiproliferative effects on various human cancer cell lines. Its IC50 values were estimated to be 0.29 mM on HepG2, 0.30 mM on HeLa, 0.30 mM on HL60, 0.33 mM on HT-29, and 0.25 mM on AGS cells. Interestingly, HY558 demonstrated no antiproliferative effect with normal lymphocytes used as the control, and a low level of inhibition on the proliferation of A549 cancer cells. A flow cytometric analysis of HepG2 cells revealed an appreciable arrest of cells at the G1 and G2/M phases of the cell cycle following treatment with HY558. Furthermore, DNA fragmentation due to apoptosis was observed in HeLa cells treated with 0.46 mM of HY558.
Keyword
cell cycle inhibitorHY558penicillium minioluteum F558
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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