Medium optimization for phytase production by recombinant Escherichia coli using statistical experimental design

Cited 0 time in scopus
Metadata Downloads
Title
Medium optimization for phytase production by recombinant Escherichia coli using statistical experimental design
Author(s)
Won Chan Choi; Byung Chul Oh; Hyung Kwoun Kim; Eun Sook Lee; Tae Kwang Oh
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 12, no. 3, pp. 490-496
Publication Year
2002
Abstract
The production of E. coli WC7 phytase from a recombinant E. coli strain was optimized using a statistical experimental design approach. Two-level complete factorial designs with seven variables were used for the media optimization. In the first optimization step, the influence of disodium succinate, yeast extract, K2HPO4, NH4H2PO4, MgSO4, NaCl, and trace elements on phytase production was evaluated. As a result, disodium succinate, yeast extract, NH4H2PO4, NaCl, and the trace elements were found to have a positive influence on the phytase production, while K2HPO4 and MgSO4 had a negative influence. In the second step, the concentrations of disodium succinate and yeast extract were further optimized using central composite designs. The maximum phytase activity obtained was 234 U/ml using 15.9 g/l disodium succinate, 20 g/l yeast extract, 5 g/lK2HPO4, 10 g/l NH4H2PO4, 1.5 g/l MgSO4, 4 g/l NaCl, and 1.5 ml/l trace elements, which was about a 14-fold increase in comparison with that obtained using the basal medium.
Keyword
phytase productionE. colimedium optimizationrecombinant proteinexperimental designs
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.