DC Field | Value | Language |
---|---|---|
dc.contributor.author | Do Young Kim | - |
dc.contributor.author | Ji Hye Yun | - |
dc.contributor.author | Hyung Woo Kim | - |
dc.contributor.author | Kyung Sook Bae | - |
dc.contributor.author | Young Ha Rhee | - |
dc.date.accessioned | 2017-04-19T08:59:08Z | - |
dc.date.available | 2017-04-19T08:59:08Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 1225-8873 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/5868 | - |
dc.description.abstract | The fungus, Emericellopsis minima W2, capable of degrading poly(3-hydroxybutyrate) (PHB) was isolated from a waste water sample. Production of the PHB depolymerase from E. minima W2 (PhaZEmi) was significantly repressed in the presence of glucose. PhaZEmi was purified by column chromatography on Octyl-Sepharose CL-4B and Sephadex G-100. The molecular mass of the PhaZEmi, which consisted of a single polypeptide chain, was estimated to be 48.0 kDa by SDS-PAGE and its pI value was 4.4. The maximum activity of the PhaZEmi was observed at pH 9.0 and 55°C. It was significantly inactivated by 1 mM dithiothreitol, 2 mM diisopropyl fluorophosphate, 0.1 mM Tween 80, and 0.1 mM Triton X-100, but insensitive to phenylmethylsulfonyl fluoride and N-ethylmaleimide. The PhaZEmi efficiently hydrolyzed PHB and its copolyester with 30 mol% 3-hydroxyvalerate, but did not act on poly(3-hydroxyoctanoate). It also hydrolyzed p-nitrophenylacetate and p-nitrophenylbutyrate but hardly affected the longer-chain forms. The main hydrolysis product of PHB was identified as a dimer of 3-hydroxybutyrate. | - |
dc.publisher | Microbiological Society Korea | - |
dc.title | Purification and characterization of poly(3-hydroxylbutyrate) depolymerase from a fungal isolate, Emericellopsis minima W2 | - |
dc.title.alternative | Purification and characterization of poly(3-hydroxylbutyrate) depolymerase from a fungal isolate, Emericellopsis minima W2 | - |
dc.type | Article | - |
dc.citation.title | Journal of Microbiology | - |
dc.citation.number | 2 | - |
dc.citation.endPage | 133 | - |
dc.citation.startPage | 129 | - |
dc.citation.volume | 40 | - |
dc.contributor.affiliatedAuthor | Do Young Kim | - |
dc.contributor.affiliatedAuthor | Kyung Sook Bae | - |
dc.contributor.alternativeName | 김도영 | - |
dc.contributor.alternativeName | 윤지혜 | - |
dc.contributor.alternativeName | 김형우 | - |
dc.contributor.alternativeName | 배경숙 | - |
dc.contributor.alternativeName | 이영하 | - |
dc.identifier.bibliographicCitation | Journal of Microbiology, vol. 40, no. 2, pp. 129-133 | - |
dc.subject.keyword | emericellopsis minima | - |
dc.subject.keyword | PHB depolymerase | - |
dc.subject.keyword | poly(3-hydroxyalkanoate) | - |
dc.subject.keyword | poly(3-hydroxybutyrate) | - |
dc.subject.local | emericellopsis minima | - |
dc.subject.local | PHB depolymerase | - |
dc.subject.local | poly(3-hydroxyalkanoate) | - |
dc.subject.local | poly(3-hydroxybutyrate) | - |
dc.subject.local | Poly(3-hydroxybutyrate) | - |
dc.description.journalClass | Y | - |
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