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- Carnitine uptake by AGP2 in yeast Saccharomyces cerevisiae is dependent on Hog1 MAP kinase pathway
- Jiyoung Lee; Bo Young Lee; Dong Jin Shin; Sang Soo Kwak; Jeong Dong Bahk; Chae Oh Lim; Dae Jin Yun
- Bibliographic Citation
- Molecules and Cells, vol. 13, no. 3, pp. 407-412
- Publication Year
- The AGP2 gene encodes a plasma membrane carnitine transporter in S. cerevisiae. Here, we report the identification of AGP2 as an osmotic stress response gene. AGP2 was isolated from mTn3 tagged mutants that contained in-frame fusions with lacZ. The expression of AGP2 was down-regulated by osmotic stresses, including NaCl, sorbitol, and KCl. We also found that carnitine uptake was inhibited by NaCl. In the ssk1Δ ste11Δ double-mutant strain, the expression of AGP2 and the uptake of carnitine were greatly reduced compared to the wild-type strain. Furthermore, carnitine uptake was inhibited by the constitutive expression of PBS2, which encodes a MAPKK that activates Hog1. We concluded, therefore, that the HOG pathway plays an important role in the regulation of carnitine uptake in S. cerevisiae.
- carnitine uptake; expression; HOG Pathway; osmotic stress; S. cerevisiae
- South Korea
- Appears in Collections:
- Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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