Carnitine uptake by AGP2 in yeast Saccharomyces cerevisiae is dependent on Hog1 MAP kinase pathway

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Title
Carnitine uptake by AGP2 in yeast Saccharomyces cerevisiae is dependent on Hog1 MAP kinase pathway
Author(s)
Jiyoung Lee; Bo Young Lee; Dong Jin Shin; Sang Soo Kwak; Jeong Dong Bahk; Chae Oh Lim; Dae Jin Yun
Bibliographic Citation
Molecules and Cells, vol. 13, no. 3, pp. 407-412
Publication Year
2002
Abstract
The AGP2 gene encodes a plasma membrane carnitine transporter in S. cerevisiae. Here, we report the identification of AGP2 as an osmotic stress response gene. AGP2 was isolated from mTn3 tagged mutants that contained in-frame fusions with lacZ. The expression of AGP2 was down-regulated by osmotic stresses, including NaCl, sorbitol, and KCl. We also found that carnitine uptake was inhibited by NaCl. In the ssk1Δ ste11Δ double-mutant strain, the expression of AGP2 and the uptake of carnitine were greatly reduced compared to the wild-type strain. Furthermore, carnitine uptake was inhibited by the constitutive expression of PBS2, which encodes a MAPKK that activates Hog1. We concluded, therefore, that the HOG pathway plays an important role in the regulation of carnitine uptake in S. cerevisiae.
Keyword
carnitine uptakeexpressionHOG Pathwayosmotic stressS. cerevisiae
ISSN
1016-8478
Publisher
South Korea
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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