DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ha Ryoung Poo | - |
dc.contributor.author | Jae Jun Song | - |
dc.contributor.author | Seung Pyo Hong | - |
dc.contributor.author | Yoon Ho Choi | - |
dc.contributor.author | Sun Woo Yoon | - |
dc.contributor.author | Jin Ho Kim | - |
dc.contributor.author | Sang Chul Lee | - |
dc.contributor.author | Seung Goo Lee | - |
dc.contributor.author | Moon Hee Sung | - |
dc.date.accessioned | 2017-04-19T08:59:10Z | - |
dc.date.available | 2017-04-19T08:59:10Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | 10.1023/A:1016107230825 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/5878 | - |
dc.description.abstract | A new E. coli expression system, the pHCE-IIB vector, using a constitutively expressing HCE promoter derived upstream from the D-amino acid aminotransferase (D-AAT) gene of Geobacillus toebii, was developed for the high-level expression of foreign proteins without induction. The expression efficiency of recombinant human tumor necrosis factor-α (rhTNFα) using pHCE-IIB was compared with that of the pET14B vector under the control of a T7 promoter induced by IPTG. The total amount of rhTNFα produced by the pHCE system was approximately twice that produced by the pET system under optimal fermentation conditions thereby demonstrating the convenience and economical advantage of the new constitutive pHCE-IIB expression vector over the conventional pET expression vector. | - |
dc.publisher | Springer | - |
dc.title | Novel high-level constitutive expression system, pHCE vector, for a convenient and cost-effective soluble production of human tumor necrosis factor-α | - |
dc.title.alternative | Novel high-level constitutive expression system, pHCE vector, for a convenient and cost-effective soluble production of human tumor necrosis factor-α | - |
dc.type | Article | - |
dc.citation.title | Biotechnology Letters | - |
dc.citation.number | 14 | - |
dc.citation.endPage | 1189 | - |
dc.citation.startPage | 1185 | - |
dc.citation.volume | 24 | - |
dc.contributor.affiliatedAuthor | Ha Ryoung Poo | - |
dc.contributor.affiliatedAuthor | Jae Jun Song | - |
dc.contributor.affiliatedAuthor | Seung Pyo Hong | - |
dc.contributor.affiliatedAuthor | Yoon Ho Choi | - |
dc.contributor.affiliatedAuthor | Sun Woo Yoon | - |
dc.contributor.affiliatedAuthor | Jin Ho Kim | - |
dc.contributor.affiliatedAuthor | Sang Chul Lee | - |
dc.contributor.affiliatedAuthor | Seung Goo Lee | - |
dc.contributor.affiliatedAuthor | Moon Hee Sung | - |
dc.contributor.alternativeName | 부하령 | - |
dc.contributor.alternativeName | 송재준 | - |
dc.contributor.alternativeName | 홍승표 | - |
dc.contributor.alternativeName | 최윤호 | - |
dc.contributor.alternativeName | 윤선우 | - |
dc.contributor.alternativeName | 김진호 | - |
dc.contributor.alternativeName | 이상철 | - |
dc.contributor.alternativeName | 이승구 | - |
dc.contributor.alternativeName | 성문희 | - |
dc.identifier.bibliographicCitation | Biotechnology Letters, vol. 24, no. 14, pp. 1185-1189 | - |
dc.identifier.doi | 10.1023/A:1016107230825 | - |
dc.subject.keyword | Constitutive expression | - |
dc.subject.keyword | Isopropyl-beta-D-thiogalactosidase | - |
dc.subject.keyword | PHCE | - |
dc.subject.keyword | Recombinant human tumor necrosis factor | - |
dc.subject.local | constitutive expression | - |
dc.subject.local | Constitutive expression | - |
dc.subject.local | Isopropyl-beta-D-thiogalactosidase | - |
dc.subject.local | PHCE | - |
dc.subject.local | Recombinant human tumor necrosis factor | - |
dc.description.journalClass | Y | - |
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