Novel high-level constitutive expression system, pHCE vector, for a convenient and cost-effective soluble production of human tumor necrosis factor-α

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dc.contributor.authorHa Ryoung Poo-
dc.contributor.authorJae Jun Song-
dc.contributor.authorSeung Pyo Hong-
dc.contributor.authorYoon Ho Choi-
dc.contributor.authorSun Woo Yoon-
dc.contributor.authorJin Ho Kim-
dc.contributor.authorSang Chul Lee-
dc.contributor.authorSeung Goo Lee-
dc.contributor.authorMoon Hee Sung-
dc.date.accessioned2017-04-19T08:59:10Z-
dc.date.available2017-04-19T08:59:10Z-
dc.date.issued2002-
dc.identifier.issn0141-5492-
dc.identifier.uri10.1023/A:1016107230825ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5878-
dc.description.abstractA new E. coli expression system, the pHCE-IIB vector, using a constitutively expressing HCE promoter derived upstream from the D-amino acid aminotransferase (D-AAT) gene of Geobacillus toebii, was developed for the high-level expression of foreign proteins without induction. The expression efficiency of recombinant human tumor necrosis factor-α (rhTNFα) using pHCE-IIB was compared with that of the pET14B vector under the control of a T7 promoter induced by IPTG. The total amount of rhTNFα produced by the pHCE system was approximately twice that produced by the pET system under optimal fermentation conditions thereby demonstrating the convenience and economical advantage of the new constitutive pHCE-IIB expression vector over the conventional pET expression vector.-
dc.publisherSpringer-
dc.titleNovel high-level constitutive expression system, pHCE vector, for a convenient and cost-effective soluble production of human tumor necrosis factor-α-
dc.title.alternativeNovel high-level constitutive expression system, pHCE vector, for a convenient and cost-effective soluble production of human tumor necrosis factor-α-
dc.typeArticle-
dc.citation.titleBiotechnology Letters-
dc.citation.number14-
dc.citation.endPage1189-
dc.citation.startPage1185-
dc.citation.volume24-
dc.contributor.affiliatedAuthorHa Ryoung Poo-
dc.contributor.affiliatedAuthorJae Jun Song-
dc.contributor.affiliatedAuthorSeung Pyo Hong-
dc.contributor.affiliatedAuthorYoon Ho Choi-
dc.contributor.affiliatedAuthorSun Woo Yoon-
dc.contributor.affiliatedAuthorJin Ho Kim-
dc.contributor.affiliatedAuthorSang Chul Lee-
dc.contributor.affiliatedAuthorSeung Goo Lee-
dc.contributor.affiliatedAuthorMoon Hee Sung-
dc.contributor.alternativeName부하령-
dc.contributor.alternativeName송재준-
dc.contributor.alternativeName홍승표-
dc.contributor.alternativeName최윤호-
dc.contributor.alternativeName윤선우-
dc.contributor.alternativeName김진호-
dc.contributor.alternativeName이상철-
dc.contributor.alternativeName이승구-
dc.contributor.alternativeName성문희-
dc.identifier.bibliographicCitationBiotechnology Letters, vol. 24, no. 14, pp. 1185-1189-
dc.identifier.doi10.1023/A:1016107230825-
dc.subject.keywordConstitutive expression-
dc.subject.keywordIsopropyl-beta-D-thiogalactosidase-
dc.subject.keywordPHCE-
dc.subject.keywordRecombinant human tumor necrosis factor-
dc.subject.localconstitutive expression-
dc.subject.localConstitutive expression-
dc.subject.localIsopropyl-beta-D-thiogalactosidase-
dc.subject.localPHCE-
dc.subject.localRecombinant human tumor necrosis factor-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > 1. Journal Articles
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