Introduction of VP6 gene into potato plant by Agrobacterium-mediated transformation and analysis of VP6 expression in transgenic potatoes = Rotavirus VP6 유전자의 감자식물체내로의 도입과 형질전환체의 발현분석

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dc.contributor.authorJung Won Youm-
dc.contributor.authorJae Heung Jeon-
dc.contributor.authorJae Yeol Jung-
dc.contributor.authorByoung Chan Lee-
dc.contributor.authorWon Jin Kang-
dc.contributor.authorMi Sun Kim-
dc.contributor.authorChul Joong Kim-
dc.contributor.authorHyouk Joung-
dc.contributor.authorHyun Soon Kim-
dc.date.accessioned2017-04-19T08:59:11Z-
dc.date.available2017-04-19T08:59:11Z-
dc.date.issued2002-
dc.identifier.issn1015-5880-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5881-
dc.description.abstractA VP6 fragments was subcloned with BamHI in the binary pMBP-1 vector under Califlower Mosaic Virus (CaMV) 355 promoter and neomycin phosphotransferase II (npt II) gene. The recombinant binary vector was mobilized into Agrobacterium-tumefaciens LBA4404 by the freeze-thaw method and potato (Solanum tubensum L. cv Desiree) was transformed by modified leaf-disc cocultivation. Shoots were induced on MS medium with 0.01 mg/L NAA, 0.1 mg/L GA_3, 2.0 mg/L Zeatin, 100.0 mg/L kanamycin, 500.0 mg/L carbenicillin. In order to identify the copy number of VP6 into potato plant, total genomic DNA was isolated from transgenic potato and analysed by Southern blotting. Genomic DNA and total mRNA analysis demonstrated the incorporation of the foreign gene into the potato genome, as well as their transcription.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleIntroduction of VP6 gene into potato plant by Agrobacterium-mediated transformation and analysis of VP6 expression in transgenic potatoes = Rotavirus VP6 유전자의 감자식물체내로의 도입과 형질전환체의 발현분석-
dc.title.alternativeIntroduction of VP6 gene into potato plant by Agrobacterium-mediated transformation and analysis of VP6 expression in transgenic potatoes-
dc.typeArticle-
dc.citation.titleKorean Journal of Plant Biotechnology (Tissue Culture)-
dc.citation.number2-
dc.citation.endPage98-
dc.citation.startPage93-
dc.citation.volume29-
dc.contributor.affiliatedAuthorJung Won Youm-
dc.contributor.affiliatedAuthorJae Heung Jeon-
dc.contributor.affiliatedAuthorJae Yeol Jung-
dc.contributor.affiliatedAuthorByoung Chan Lee-
dc.contributor.affiliatedAuthorWon Jin Kang-
dc.contributor.affiliatedAuthorMi Sun Kim-
dc.contributor.affiliatedAuthorHyouk Joung-
dc.contributor.affiliatedAuthorHyun Soon Kim-
dc.contributor.alternativeName염정원-
dc.contributor.alternativeName전재흥-
dc.contributor.alternativeName정재열-
dc.contributor.alternativeName이병찬-
dc.contributor.alternativeName강원진-
dc.contributor.alternativeName김미선-
dc.contributor.alternativeName김철중-
dc.contributor.alternativeName정혁-
dc.contributor.alternativeName김현순-
dc.identifier.bibliographicCitationKorean Journal of Plant Biotechnology (Tissue Culture), vol. 29, no. 2, pp. 93-98-
dc.identifier.doi10.5010/JPB.2002.29.2.093-
dc.subject.keywordsolanum tuberosum L. cv Desiree-
dc.subject.keywordVP6 gene-
dc.subject.keywordCaMV 35S promoter-
dc.subject.keywordNPT Ⅱ-
dc.subject.localsolanum tuberosum L. cv Desiree-
dc.subject.localsolanum tuberosum L. cv desiree-
dc.subject.localVP6 gene-
dc.subject.localCaMV 35S promoter-
dc.subject.localNPT Ⅱ-
dc.description.journalClassN-
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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