Screening of soybean recombinant inbred lines for high competence somatic embryogenesis = 대두 재조합순계주에서 고빈도 체세포배발생능 계통 조사

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Title
Screening of soybean recombinant inbred lines for high competence somatic embryogenesis = 대두 재조합순계주에서 고빈도 체세포배발생능 계통 조사
Author(s)
Pil Sun Choi; Takao Komatsuda; Min Hoon Kim; Kyu Myeong Choi; Dong Woog Choi; Jang Ryol Liu
Bibliographic Citation
Korean Journal of Plant Biotechnology (Tissue Culture), vol. 29, no. 2, pp. 135-138
Publication Year
2002
Abstract
Cotyledonary explants from immature zygotic embryos of each 85 recombinant inbred lines (RILs) were cultured on medium containing MS salts, B5 vitamins, 40 mg^{-1} 2,4-dichlorophenoxyacetic acid and 30 g^{-1} sucrose. Frequency of somatic embryo formation on cotyledonary explants showed in thirty-six lines(10%), in thirty-seven lines (11~49%), in nine lines (50~89%), and in three lines(>90%), respectively, The highest frequency (up to 90%) and number (6.36 per cotyledon) of somatic embryos were obtained from lines of KM1010, KM1032 and KM1064. Primary somatic embryos produced from three lines produced numerous secondary somatic embryos on the surfaces, which were subcultured for over one year. Upon transfer to maturation and conversion medium (Komatsuda, 1992), somatic embryos converted to plantlets at a frequency of approximately 25%.
Keyword
soybeanglycine max L.glycine gracilis skvortz.somatic embryogenesisrecombinant inbred lines (RILs)
ISSN
1015-5880
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.5010/JPB.2002.29.2.135
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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