Sequencing and functional analysis of the Hansenula polymorpha genomic fragment containing the YPT1 and PMI40 genes

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Title
Sequencing and functional analysis of the Hansenula polymorpha genomic fragment containing the YPT1 and PMI40 genes
Author(s)
Moo Woong Kim; M O Agaphonov; J Y Kim; Sang Ki Rhee; Hyun Ah Kang
Bibliographic Citation
Yeast, vol. 19, no. 10, pp. 863-871
Publication Year
2002
Abstract
A 6.0 kb genomic DNA segment was isolated by its ability to rescue the temperature-sensitive growth defect and the hypersensitivity to sodium deoxycholate of a spontaneous vanadate-resistant mutant derived from Hansenula polymorpha DL-1. The genomic fragment contains four open reading frames homologous to the Saccharomyces cerevisiae genes YPT1 (which codes for a GTP-binding protein; 75% amino acid identity), PM140 (encoding phosphomannose isomerase; 61% identity), YLRO65c (30% identity) and CST13 (28% identity). The H. polymorpha YPT1 homologue (HpYPT1) was found to be responsible for the complementation of the temperature-sensitive phenotype and the sodium deoxycholate sensitivity of the mutant strain. Disruption of the H. polymorpha PM140 homologue (HpPMI40) resulted in the auxotrophic requirement for D-mannose. The heterologous expressions of HpYPT1 and HpPMI40 were able to complement the temperature-sensitive phenotype of S. cerevisiae ypt1-1 mutant and the mannose auxotrophy of S. cerevisiae pmi40 null mutant, respectively, indicating that the H. polymorpha genes encode the functional homologues of S. cerevisiae YPT1 and PM140 proteins. The nucleotide sequence has been submitted to GenBank under Accession No. AF454544.
Keyword
functional complementationhansenula polymorphaPM140sequence analysisYPT1
ISSN
0749-503X
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/yea.881
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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