Isolation of anticomplementary substances from Cucurbita moschata Duch

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Isolation of anticomplementary substances from Cucurbita moschata Duch
J O Yang; Sei Ryang Oh; Hyeong Kyu Lee; Chang Jin Kim; K B Song
Bibliographic Citation
Journal of Food Science, vol. 67, no. 4, pp. 1348-1351
Publication Year
Chloroform and ethyl acetate extracts of the edible part of Cucurbita moschata Duch, which is commonly used as an oriental medicine as well as a popular food source, showed significant anticomplementary activities on the classical pathway of the complement system. Bioassay-guided chromatographic separation using silica gel, Sephadex LH-20, octadecyl silica gel, thin layer chromatography, and high performance liquid chromatography was performed to isolate the anticomplementary substances. Among the isolated substances, apigenin, phytosterols, and mixture of fatty acids, which were identified by nuclear magnetic resonance and gas chromatography-mass spectrometry, presented the strongest activities. Especially, phytosterols and mixture of saturated fatty acids isolated from the ethyl acetate extracts had 0.74 and 0.67 mg/ml as IC50, respectively, indicating that the ethyl acetate extraction is the best way to isolate the anticomplementary substances from Cucurbita moschata Duch.
anticomplementary substancesextractsisolation
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Ochang Branch Institute > 1. Journal Articles
Division of Biomaterials Research > Industrial Bio-materials Research Center > 1. Journal Articles
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