Hepatitis C virus core protein represses the p21 promoter through inhibition of a TGF-β pathway

Cited 39 time in scopus
Metadata Downloads
Title
Hepatitis C virus core protein represses the p21 promoter through inhibition of a TGF-β pathway
Author(s)
M N Lee; E Y Jung; H J Kwun; H K Jun; Dae Yeul Yu; Y H Choi; K L Jang
Bibliographic Citation
Journal of General Virology, vol. 83, no. 9, pp. 2145-2151
Publication Year
2002
Abstract
The increased proliferation rate of hepatocytes is one of the major risk factors for the development of hepatocellular carcinoma. In this study, we investigated the mechanism by which hepatitis C virus (HCV) core protein represses transcription of the universal cyclin-dependent kinase inhibitor p21 gene in murine fibroblast NIH 3T3 cells. From the transient reporter assays of p2l promoter, we found that the TGF-β-responsive element (TβRE) located between -83 and -74 of the p2l promoter is responsible for the effect. The TGF-β-induced p21 promoter activity was specifically decreased by HCV core protein and in the presence of the inhibitory Smad7 the repression effect was almost completely abolished. Furthermore, HCV core protein stimulated the growth rate of NIH 3T3 cells and could overcome growth arrest by TGF-β but not by butyrate, suggesting that HCV core protein stimulates cell cycle progression by repressing p21 transcription through a TGF-β pathway.
ISSN
0022-1317
Publisher
Microbiology Soc
DOI
http://dx.doi.org/10.1099/0022-1317-83-9-2145
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.