Characterization of TCE-degrading bacteria and their application to wastewater treatment

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Characterization of TCE-degrading bacteria and their application to wastewater treatment
Wan Seok Lee; Chan Sun Park; J E Kim; Byung Dae Yoon; Hee Mock Oh
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 12, no. 4, pp. 569-575
Publication Year
Two bacterial strains capable of degrading trichloroethylene (TCE), isolated from soils contaminated with various chlorinated alkenes, were identified as Alcaligenes odorans N6 and Nocardia sp. H17. In addition, four KCTC strains, including three strains of Pseudomonas putida and one strain of Sphingomonas chlorophenolica, exhibited an ability to degrade toluene. A. odorans N6 and Nocardia sp. H17 degraded 84% of the initial amount of TCE in a basal salts medium (BSM), containing 0.2 mM TCE as the sole source of carbon and energy, in a day. The optimal pH for growth was within a range of 7.0-8.0. A mixed culture of the four toluene-degrading isolates degraded 95% of 0.2 mM TCE with 1.5 mM toluene as an inducer, whereas no TCE was degraded by the same mixture without an inducer. When a mixed culture of all 6 isolates was used, the degradation efficiency of 0.2 mM TCE was 72% without an inducer, in a day, and 82% with toluene as an inducer. In a continuous treatment, 1,000 mg/1 of TCE in an artificial wastewater was completely removed within 18 h when an activated sludge was used along with the microbial mixture, which was 27 h faster than when only an activated sludge was used. According, it would appear that such a microbial mixture could be effectively applied to the biological treatment of wastewater containing TCE with or without an inducer.
alcaligenes odoransmicrobial mixturenocardia sp.trichloroethylenewastewater treatmentbacterial strainbacterium identification
Korea Soc-Assoc-Inst
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Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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