DC Field | Value | Language |
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dc.contributor.author | Seung Ho Kim | - |
dc.contributor.author | S J Park | - |
dc.contributor.author | J W Lee | - |
dc.contributor.author | J Y Jin | - |
dc.date.accessioned | 2017-04-19T08:59:18Z | - |
dc.date.available | 2017-04-19T08:59:18Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 1225-8687 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/5933 | - |
dc.description.abstract | Thrombotic thrombocytopenic purpura (TTP) is characterized by widespread platelet thrombi in arterioles and capillaries. Unusually large or multimeric von Willebrand factor, as well as one or more platelet-agglutinating factors, have been implicated in the pathogenesis of TTP. But, the actual mechanisms of platelet agglutination have not been satisfactorily explained. Recent studies suggested the 37-kDa platelet-agglutinating protein (PAP) p37 to be partially responsible for the formation of platelet thrombi in patients with TTP. We studied mobility in SDS-PAGE, the sequence of N-terminal amino acid residues, DNA and antigenic characteristics of PAP p37, which might be related to the pathogenesis of TTP. PAP p37 was purified from the plasma of a 31-year-old male Korean patient with acute TTP. The findings are as follows: (1) We compared PAP p37 with thrombin through the use of SDS-PAGE, either with or without β-mercaptoethanol. PAP p37 did not appear to be cleaved between the A- and B-chains of prethrombin 2. However, thrombin did cleave between those of prethrombin 2, but linked with disulfide bridge. (2) Nterminal 21 amino acid sequence of PAP p37 was T-F-G-S-G-E-A-D-X-G-L-R-P-L-F-E-K-K-S-L-E. It appeared to be identical to that of 285-305 amino acid residues of human prothrombin (prethrombin 2). (3) No prothrombin gene DNA mutation was revealed. (4). The antigenicity of PAP p37 was similar to thrombin, which was a result of the competitive binding against the anti-thrombin antibody. With these results, we conclude that PAP p37 has similar characteristics to prethrombin2. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Platelet-agglutinating protein p37 from a patient with thrombotic thrombocytopenic purpura has characteristics similar to prethrombin 2 | - |
dc.title.alternative | Platelet-agglutinating protein p37 from a patient with thrombotic thrombocytopenic purpura has characteristics similar to prethrombin 2 | - |
dc.type | Article | - |
dc.citation.title | BMB Reports | - |
dc.citation.number | 5 | - |
dc.citation.endPage | 531 | - |
dc.citation.startPage | 524 | - |
dc.citation.volume | 35 | - |
dc.contributor.affiliatedAuthor | Seung Ho Kim | - |
dc.contributor.alternativeName | 김승호 | - |
dc.contributor.alternativeName | 박수정 | - |
dc.contributor.alternativeName | 이종우 | - |
dc.contributor.alternativeName | 진종열 | - |
dc.identifier.bibliographicCitation | BMB Reports, vol. 35, no. 5, pp. 524-531 | - |
dc.identifier.doi | 10.5483/BMBRep.2002.35.5.524 | - |
dc.subject.keyword | p37 | - |
dc.subject.keyword | Platelet-agglutinating protein (PAP) | - |
dc.subject.keyword | Prethrombin2 | - |
dc.subject.keyword | TTP | - |
dc.subject.local | p37 | - |
dc.subject.local | Platelet-agglutinating protein (PAP) | - |
dc.subject.local | Prethrombin2 | - |
dc.subject.local | TTP | - |
dc.description.journalClass | Y | - |
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