Platelet-agglutinating protein p37 from a patient with thrombotic thrombocytopenic purpura has characteristics similar to prethrombin 2

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dc.contributor.authorSeung Ho Kim-
dc.contributor.authorS J Park-
dc.contributor.authorJ W Lee-
dc.contributor.authorJ Y Jin-
dc.date.accessioned2017-04-19T08:59:18Z-
dc.date.available2017-04-19T08:59:18Z-
dc.date.issued2002-
dc.identifier.issn1225-8687-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5933-
dc.description.abstractThrombotic thrombocytopenic purpura (TTP) is characterized by widespread platelet thrombi in arterioles and capillaries. Unusually large or multimeric von Willebrand factor, as well as one or more platelet-agglutinating factors, have been implicated in the pathogenesis of TTP. But, the actual mechanisms of platelet agglutination have not been satisfactorily explained. Recent studies suggested the 37-kDa platelet-agglutinating protein (PAP) p37 to be partially responsible for the formation of platelet thrombi in patients with TTP. We studied mobility in SDS-PAGE, the sequence of N-terminal amino acid residues, DNA and antigenic characteristics of PAP p37, which might be related to the pathogenesis of TTP. PAP p37 was purified from the plasma of a 31-year-old male Korean patient with acute TTP. The findings are as follows: (1) We compared PAP p37 with thrombin through the use of SDS-PAGE, either with or without β-mercaptoethanol. PAP p37 did not appear to be cleaved between the A- and B-chains of prethrombin 2. However, thrombin did cleave between those of prethrombin 2, but linked with disulfide bridge. (2) Nterminal 21 amino acid sequence of PAP p37 was T-F-G-S-G-E-A-D-X-G-L-R-P-L-F-E-K-K-S-L-E. It appeared to be identical to that of 285-305 amino acid residues of human prothrombin (prethrombin 2). (3) No prothrombin gene DNA mutation was revealed. (4). The antigenicity of PAP p37 was similar to thrombin, which was a result of the competitive binding against the anti-thrombin antibody. With these results, we conclude that PAP p37 has similar characteristics to prethrombin2.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titlePlatelet-agglutinating protein p37 from a patient with thrombotic thrombocytopenic purpura has characteristics similar to prethrombin 2-
dc.title.alternativePlatelet-agglutinating protein p37 from a patient with thrombotic thrombocytopenic purpura has characteristics similar to prethrombin 2-
dc.typeArticle-
dc.citation.titleBMB Reports-
dc.citation.number5-
dc.citation.endPage531-
dc.citation.startPage524-
dc.citation.volume35-
dc.contributor.affiliatedAuthorSeung Ho Kim-
dc.contributor.alternativeName김승호-
dc.contributor.alternativeName박수정-
dc.contributor.alternativeName이종우-
dc.contributor.alternativeName진종열-
dc.identifier.bibliographicCitationBMB Reports, vol. 35, no. 5, pp. 524-531-
dc.identifier.doi10.5483/BMBRep.2002.35.5.524-
dc.subject.keywordp37-
dc.subject.keywordPlatelet-agglutinating protein (PAP)-
dc.subject.keywordPrethrombin2-
dc.subject.keywordTTP-
dc.subject.localp37-
dc.subject.localPlatelet-agglutinating protein (PAP)-
dc.subject.localPrethrombin2-
dc.subject.localTTP-
dc.description.journalClassY-
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