Molecular characterization of the growth phase-dependent expression of the lsrA gene, encoding levansucrase of Rahnella aquatilis

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dc.contributor.authorJeong Woo Seo-
dc.contributor.authorKi Hyo Jang-
dc.contributor.authorS A Kang-
dc.contributor.authorKi Bang Song-
dc.contributor.authorEun Kyung Jang-
dc.contributor.authorBuem Seek Park-
dc.contributor.authorChul Ho Kim-
dc.contributor.authorSang Ki Rhee-
dc.date.accessioned2017-04-19T08:59:19Z-
dc.date.available2017-04-19T08:59:19Z-
dc.date.issued2002-
dc.identifier.issn00219193-
dc.identifier.uri10.1128/JB.184.21.5862-5870.2002ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5937-
dc.description.abstractExpression of the lsrA gene from Rahnella aquatilis, encoding levansucrase, is tightly regulated by the growth phase of the host cell; low-level expression was observed in the early phase of cell growth, but expression was significantly stimulated in the late phase. Northern blot analysis revealed that regulation occurred at the level of transcription. The promoter region was identified by primer extension analysis. Two opposite genetic elements that participate in the regulation of lsrA expression were identified upstream of the lsrA gene: the lsrS gene and the lsrR region. The lsrS gene encodes a protein consisting of 70 amino acid residues (Mr, 8,075), which positively activated lsrA expression approximately 20-fold in a growth phase-dependent fashion. The cis-acting lsrR region, which repressed lsrA expression about 10-fold, was further narrowed to two DNA regions by deletion analysis. The concerted action of two opposite regulatory functions resulted in the growth phase-dependent activation of gene expression in Escherichia coli independent of the stationary sigma factor σs.-
dc.publisherAmer Soc Microb-
dc.titleMolecular characterization of the growth phase-dependent expression of the lsrA gene, encoding levansucrase of Rahnella aquatilis-
dc.title.alternativeMolecular characterization of the growth phase-dependent expression of the lsrA gene, encoding levansucrase of Rahnella aquatilis-
dc.typeArticle-
dc.citation.titleJournal of Bacteriology-
dc.citation.number21-
dc.citation.endPage5870-
dc.citation.startPage5862-
dc.citation.volume184-
dc.contributor.affiliatedAuthorJeong Woo Seo-
dc.contributor.affiliatedAuthorChul Ho Kim-
dc.contributor.alternativeName서정우-
dc.contributor.alternativeName장기효-
dc.contributor.alternativeName강순아-
dc.contributor.alternativeName송기방-
dc.contributor.alternativeName장은경-
dc.contributor.alternativeName박범식-
dc.contributor.alternativeName김철호-
dc.contributor.alternativeName이상기-
dc.identifier.bibliographicCitationJournal of Bacteriology, vol. 184, no. 21, pp. 5862-5870-
dc.identifier.doi10.1128/JB.184.21.5862-5870.2002-
dc.description.journalClassY-
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Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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