Isolation and characterization of a strain of Bacillus thuringiensis ssp. kurstaki containing a new δ-endotoxin gene

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Title
Isolation and characterization of a strain of Bacillus thuringiensis ssp. kurstaki containing a new δ-endotoxin gene
Author(s)
M S Li; Y H Je; I H Lee; J H Chang; J Y Roh; H S Kim; Hyun Woo Oh; K S Boo
Bibliographic Citation
Current Microbiology, vol. 45, no. 4, pp. 299-302
Publication Year
2002
Abstract
A strain of Bacillus thuringiensis that showed significantly high toxicity to Plutella xylostella and Spodoptera exigua was isolated from a Korean soil sample and characterized. The isolate, named B. thuringiensis K1, was determined to belong to ssp. kurstaki (H3a3b3c) type by an H antisera agglutination test and produced bipyramidal inclusions. Plasmid pattern of K1 was different from that of the reference strain, ssp. kurstaki HD-1, but the parasporal inclusion protein profile of K1 had two major bands that were similar in size to those of ssp. kurstaki HD-1. To verify the δ-endotoxin gene types of K1, PCR analysis with specific cry gene primers was performed to show that K1 contained a new cry gene in addition to cry1Aa, cry1Ab, cry1Ac, cry1E and cry2 genes. PCR-amplified region of the new cry gene, cpyX, showed 79% similarity to cry1Fa1 gene (GenBank Accession No. M63897). In an insect toxicity assay, K1 had higher toxicity against Plutella xylostella and S. exigua than ssp. kurstaki HD-1.
ISSN
0343-8651
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s00284-002-3755-0
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > Core Research Facility & Analysis Center > 1. Journal Articles
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