서로 다른 urokinase type plasminogen activator(uPA)와 uPA receptor(uPAR) 발현 양상을 보이는 사람 위암세포주의 종양 형성능 비교

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Title
서로 다른 urokinase type plasminogen activator(uPA)와 uPA receptor(uPAR) 발현 양상을 보이는 사람 위암세포주의 종양 형성능 비교
Author(s)
S Y Woo; B N Park; Yang Kyu Choi; M A Lyu; I A Park; Byung Hwa Hyun; Y H Kook
Bibliographic Citation
Korean Journal of Thrombosis & Hemostasis, vol. 7, no. 2, pp. 99-108
Publication Year
2000
Abstract
Background: Urokinase type plasminogen activator (uPA) is a serine protease converting plasminogen to plasmin and has been known to be involved in several physiological and pathological functions including tissue remodeling, wound healing, new blood vessel formation (angiogenesis), tumor cell invasion and metastasis. uPA is secreted as an inactive precursor form and binds to its own cell surface receptor (uPAR), which is required for the activation of inactive uPA. The expression level of uPAR is highly associated with uPA activity. Furthermore, increased expression of both molecules on many cancer cells is related to their invasion and metastasis. It was previously reported that different expression levels of uPA and uPAR, also determined invasiveness and tumorigenicity of human gastric cancer cell lines in vivo. However, those results had limitations because of the short-term (1 week) observation on chorioallantoic membrane. So we examined the tumorigenicity of gastric cancer cell lines showing different expression level in uPA and uPAR in Severe Combined Immunodeficient (SCID) mouse for a long-term observation. Methods: In order to confirm and estimate the expression of uPA and uPAR, cell surface uPAR level of two gastric cancer cell lines (Hs746T and SNU-5) were analyzed by flow-cytometric analysis. Tumorigenicity of both cell lines was observed in SCID mice. Tumors formed on the back of SCID mice were surgically removed and observed for the gross and microscopic characteristics. Results: Hs746T cell lines showed higher expression level of surface uPAR than SNU-5 suggesting more pericellular proteolytic activity on cell surface. Both cell lines induced tumors in SCID mice. However, the growth of Hs746T tumors was rapider than SNU-5 tumors. Furthermore Hs746T tumors showed higher rate of recurrence and metastasis than SNU-5 after surgical excision. SNU-5 tumors were soft and had large amount of mucous materials surrounding tumor mass. They had necrotic areas in central zone. Conclusion: Results described above may lead us to conclude that the cell surface proteolytic activity in terms of the level of uPA and uPAR expression in human gastric cancer cell lines might influence on the tumor growth and vessel formation.
Keyword
uPAuPARtumorigenicitySCID mouseuPAR
ISSN
I000-0151
Publisher
South Korea
Type
Article
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1. Journal Articles > Journal Articles
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