Brine shrimp lethality of the compounds from Phryma leptostachya L.

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Title
Brine shrimp lethality of the compounds from Phryma leptostachya L.
Author(s)
Sang Myung Lee; Byung Sun Min; Yung Hee Kho
Bibliographic Citation
Archives of Pharmacal Research, vol. 25, no. 5, pp. 652-654
Publication Year
2002
Abstract
Brine shrimp assay-guided fractionation and isolation of the EtOAc soluble fraction of Phryma leptostachya L. (Phrymacaceae) gave two active compounds, phrymarolin II (1) and ursolic acid (2), which were identified by physicochemical and spectroscopic methods. Compound 1 exhibited potent lethality with LD50 value of 0.0013 μg/ml, whereas 2 showed moderate lethality with LD50 value of 27.0 μg/ml against brine shrimp. The cytotoxic activities of 1 and 2 were also evaluated against one murine and five human cancer cell lines employing the sulforhodamin B (SRB) method. Compound 2 exhibited cytotoxic activity against L1210 and SK-MEL-2 cells with ED 50 values of 3.70 and 9.27 mg/ml, respectively, whereas 1 was devoid of any cytotoxic activity against all cancer cells tested.
Keyword
Brine shrimpCytotoxicityPhryma leptostachya L.Phrymarolin IIUrsolic acid
ISSN
0253-6269
Publisher
Pharmaceutical Soc Korea
DOI
http://dx.doi.org/10.1007/BF02976939
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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