Interleukin-12 p40 gene expression is induced in lipopolysaccharide-activated pituitary glands in vivo

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Title
Interleukin-12 p40 gene expression is induced in lipopolysaccharide-activated pituitary glands in vivo
Author(s)
Young Yang; Seung Hyun Han; H Kim; C M Kim; Kun Yong Kim; Sun Mi Shin; In Pyo Choi; K H Pyun
Bibliographic Citation
Neuroendocrinology, vol. 75, no. 6, pp. 347-357
Publication Year
2002
Abstract
Proinflammatory cytokines have several functions including activation of the hypothalamo-pituitary-adrenal (HPA) axis and regulation of the immune system. The present study focuses on the regulation of interleukin 12 (IL-12) and its receptor gene expression in the HPA axis under artificially induced immune stress, brought on by administration of lipopolysaccharide (LPS) to Sprague-Dawley (SD) rats. RT-PCR analyses showed that expression of the IL-12 p40 gene was significantly increased and peaked at 2 h in the pituitary gland, but not in the hypothalamus. LPS-induced IL-12 p40 gene induction in the pituitary gland was suppressed after β-adrenoceptor agonist pretreatment in vivo. Both IL-12 p40 gene induction and IL-12 production were also observed when freshly isolated pituitary glands from non-treated SD rats were incubated with LPS in vitro. Furthermore, CD14, which is known as a LPS receptor, was found to be expressed in the pituitary gland. Gel mobility shift assays using nuclear extracts prepared from the pituitary glands of rats administered LPS showed induction of NF-κB and AP-1 DNA-binding activity. These results suggest that LPS stimulates the pituitary gland directly in vivo to increase IL-12 p40 gene expression and IL-12 protein production.
Keyword
Hypothalamo-pituitary-adrenal axisInterleukinsCatecholamine agonistsLipopolysaccharideNeuroimmune interactions
ISSN
0028-3835
Publisher
Karger
DOI
http://dx.doi.org/10.1159/000059431
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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