DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ki-Young Lee | - |
dc.contributor.author | H G Kim | - |
dc.contributor.author | Mi Ran Hwang | - |
dc.contributor.author | Jung Il Chae | - |
dc.contributor.author | J M Yang | - |
dc.contributor.author | Y C Lee | - |
dc.contributor.author | Y K Choo | - |
dc.contributor.author | Young Ik Lee | - |
dc.contributor.author | S S Lee | - |
dc.contributor.author | Su Il Do | - |
dc.date.accessioned | 2017-04-19T08:59:27Z | - |
dc.date.available | 2017-04-19T08:59:27Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | 10.1074/jbc.M209618200 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/5986 | - |
dc.description.abstract | The mammalian Galβ1,3GalNAc-specific α2,3-sialyltransferase (ST3Gal I) was expressed as a secreted glycoprotein in High Five™ (Trichoplusia ni) cells. Using this recombinant ST3Gal I, we screened the synthetic hexapeptide combinatorial library to explore a sialyltransferase inhibitor. We found that the hexapeptide, NH2-GNWWWW, exhibited the most strong inhibition of ST3Gal I among five different hexapeptides that were finally selected. The kinetic analysis of ST3Gal I inhibition demonstrated that this hexapeptide could act as a competitive inhibitor (Ki = 1.1 μm) on CMP-NeuAc binding to the enzyme. Moreover, the hexapeptide was shown to strongly inhibit both N-glycan-specific α2,3and α2,6-sialyltranferase in vitro, suggesting that this peptide may inhibit the broad range of sialyltransferases regardless of their linkage specificity. The inhibitory activity in vivo was investigated by RCA-I lectin blot analyses and by metabolic D-[6-3H]GlcNH2 radiolabeling analyses of N- and O-linked oligosaccharides in Chines hamster ovary cells. Our results demonstrate that the hexapeptide can act as a generic inhibitor of the N- and O-glycan-specific sialyltransferases in mammalian cells, which results in the significantly reduced NeuAc expression on cellular glycoproteins in vivo. | - |
dc.publisher | Elsevier | - |
dc.title | The hexapeptide inhibitor of Galβ1,3GalNAc-specific α2,3-Sialyltransferase as a generic inhibitor of sialyltransferases | - |
dc.title.alternative | The hexapeptide inhibitor of Galβ1,3GalNAc-specific α2,3-Sialyltransferase as a generic inhibitor of sialyltransferases | - |
dc.type | Article | - |
dc.citation.title | Journal of Biological Chemistry | - |
dc.citation.number | 51 | - |
dc.citation.endPage | 49351 | - |
dc.citation.startPage | 49341 | - |
dc.citation.volume | 277 | - |
dc.contributor.affiliatedAuthor | Ki-Young Lee | - |
dc.contributor.affiliatedAuthor | Mi Ran Hwang | - |
dc.contributor.affiliatedAuthor | Jung Il Chae | - |
dc.contributor.affiliatedAuthor | Young Ik Lee | - |
dc.contributor.affiliatedAuthor | Su Il Do | - |
dc.contributor.alternativeName | 이기영 | - |
dc.contributor.alternativeName | 김형구 | - |
dc.contributor.alternativeName | 황미란 | - |
dc.contributor.alternativeName | 채정일 | - |
dc.contributor.alternativeName | 양재명 | - |
dc.contributor.alternativeName | 이영춘 | - |
dc.contributor.alternativeName | 추영국 | - |
dc.contributor.alternativeName | 이영익 | - |
dc.contributor.alternativeName | 이상수 | - |
dc.contributor.alternativeName | 도수일 | - |
dc.identifier.bibliographicCitation | Journal of Biological Chemistry, vol. 277, no. 51, pp. 49341-49351 | - |
dc.identifier.doi | 10.1074/jbc.M209618200 | - |
dc.description.journalClass | Y | - |
There are no files associated with this item.
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.