Transformation using in vivo and in vitro methylation in Streptomyces griseus

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dc.contributor.authorJang Yul Kwak-
dc.contributor.authorH Jiang-
dc.contributor.authorK E Kendrick-
dc.date.accessioned2017-04-19T08:59:28Z-
dc.date.available2017-04-19T08:59:28Z-
dc.date.issued2002-
dc.identifier.issn0378-1097-
dc.identifier.uri10.1016/S0378-1097(02)00512-8ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5994-
dc.description.abstractStreptomyces griseus does not readily take up foreign DNA isolated from other Streptomyces species or Escherichia coli, presumably due to its unique restriction-modification systems that function as a barrier for interspecific DNA transfer. To efficiently transform S. griseus by avoiding the restriction barriers, we methylated incoming DNA in vivo and in vitro and treated protoplasts with heat prior to transformation. Whereas heat treatment of protoplasts or methylation of the E. coli-Streptomyces shuttle vectors (pXE4 and pKK1443) did not prominently improve the transformation efficiency, HpaII methylation of the vectors from any E. coli strains tested in this study highly increased the transformation efficiency. The highest transformation efficiency was observed when the shuttle vectors were isolated from the dam, hsd strain of E. coli (GM161) and methylated by AluI and HpaII methyltransferases, and the efficiency was approximately the same as that of the vectors from S. griseus. We identified several restriction-modification systems that decrease the transformation efficiency. This research also led us to understand methylation profiles and restriction-modification systems in S. griseus.-
dc.publisherOxford Univ Press-
dc.titleTransformation using in vivo and in vitro methylation in Streptomyces griseus-
dc.title.alternativeTransformation using in vivo and in vitro methylation in Streptomyces griseus-
dc.typeArticle-
dc.citation.titleFEMS Microbiology Letters-
dc.citation.number2-
dc.citation.endPage248-
dc.citation.startPage243-
dc.citation.volume209-
dc.contributor.affiliatedAuthorJang Yul Kwak-
dc.contributor.alternativeName곽장열-
dc.contributor.alternativeNameJiang-
dc.contributor.alternativeNameKendrick-
dc.identifier.bibliographicCitationFEMS Microbiology Letters, vol. 209, no. 2, pp. 243-248-
dc.identifier.doi10.1016/S0378-1097(02)00512-8-
dc.subject.keywordmethylation-
dc.subject.keywordrestriction-modification enzyme-
dc.subject.keywordstreptomyces griseus-
dc.subject.keywordtransformation-
dc.subject.localmethylation-
dc.subject.localMethylation-
dc.subject.localrestriction-modification enzyme-
dc.subject.localstreptomyces griseus-
dc.subject.localTransformation-
dc.subject.localtransformation-
dc.description.journalClassY-
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