Cloning and characterization of the cDNA encoding the masquerade-like serine proteinase homologue gene of the silkworm, Bombyx mori

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dc.contributor.authorDoo Sang Park-
dc.contributor.authorHo Yong Park-
dc.date.accessioned2017-04-19T08:59:29Z-
dc.date.available2017-04-19T08:59:29Z-
dc.date.issued2002-
dc.identifier.issn1011-9493-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5999-
dc.description.abstractFrom Bombyx mori larvae, RT-PCR and cDNA library screening isolated masquerade-like serine proteinase homologue cDNA gene, proposed to be related to insect immunity and its characteristics were examined. The isolated gene is composed of 1.3 kb of nucleotide and 420 amino acid residues were encoded. According to the results of database search, the isolated gene showed high sequence homology with Holotrichia and Tenebrio's 45 kDa protein, Drosophila CG5390 gene. Moreover, it is composed of regulatory domain and catalytic domain, which is characteristic of serine proteinase that can be found in the insect immune reaction and embryonic development processes. Enzyme activation site by proteolytic cleavage and the sequence of three amino acids participate in the catalytic triad of enzyme and 14 cystein residues used in disulfide bridges are well conserved with the compared genes. The mRNA expression was increased following E. coli injection and constitutive expression was also observed before injection by Northern blot analysis.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleCloning and characterization of the cDNA encoding the masquerade-like serine proteinase homologue gene of the silkworm, Bombyx mori-
dc.title.alternativeCloning and characterization of the cDNA encoding the masquerade-like serine proteinase homologue gene of the silkworm, Bombyx mori-
dc.typeArticle-
dc.citation.titleKorean Journal of Entomology-
dc.citation.number3-
dc.citation.endPage131-
dc.citation.startPage125-
dc.citation.volume32-
dc.contributor.affiliatedAuthorDoo Sang Park-
dc.contributor.affiliatedAuthorHo Yong Park-
dc.contributor.alternativeName박두상-
dc.contributor.alternativeName박호용-
dc.identifier.bibliographicCitationKorean Journal of Entomology, vol. 32, no. 3, pp. 125-131-
dc.identifier.doi10.1111/j.1748-5967.2002.tb00022.x-
dc.subject.keywordinsect immunity-
dc.subject.keywordprophenoloxidase-
dc.subject.keywordserine proteinase-
dc.subject.keywordbombyx mori masquerade-like serine proteinase homologue gene-
dc.subject.localInsect immunity-
dc.subject.localinsect immunity-
dc.subject.localprophenoloxidase-
dc.subject.localPro-phenoloxidase-
dc.subject.localserine proteinase-
dc.subject.localbombyx mori masquerade-like serine proteinase homologue gene-
dc.description.journalClassN-
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
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