Amplification of mitochondrial small subunit ribosomal DNA of polypores and its potential for phylogenetic analysis

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Title
Amplification of mitochondrial small subunit ribosomal DNA of polypores and its potential for phylogenetic analysis
Author(s)
Soon Gyu Hong; Won Jin Jeong; H S Jung
Bibliographic Citation
Mycologia, vol. 94, no. 5, pp. 823-833
Publication Year
2002
Abstract
There has been a systematic need to seek adequate phylogenetic markers that can be applied in phylogenetic analyses of fungal taxa at various levels. The mitochondrial small subunit ribosomal DNA (mt SSU rDNA) is generally considered to be one of the molecules that are appropriate for phylogenetic analyses at a family level. In order to obtain universal primers for polypores of Hymenomycetes, mt SSU rRNA genes were cloned from Bjerkandera adusta, Ganoderraa lucidum, Phlebiopsis gigantea, and Phellinus laevigatus and their sequences were determined. Based on the conserved sequences of cloned genes from polypores and Agrocybe aegerita, PCR primers were designed for amplification and sequencing of mt SSU rDNAs. New primers allowed effective amplification and sequencing of almost full-sized genes from representative species of polypores and related species. Phylogenetic relationships were resolved quite efficiently by mt SSU rDNA sequences, and they proved to be more useful in phylogenetic reconstruction of Ganoderma than nuclear internal transcribed spacer (ITS) rDNA sequences.
Keyword
GanodermaHymenomycetesInformation contentUniversal primers
ISSN
0027-5514
Publisher
T&F (Taylor & Francis)
DOI
http://dx.doi.org/10.1080/15572536.2003.11833176
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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