DC Field | Value | Language |
---|---|---|
dc.contributor.author | H S Park | - |
dc.contributor.author | S J Choi | - |
dc.contributor.author | N H Park | - |
dc.contributor.author | C H Kim | - |
dc.contributor.author | Sung Uk Kim | - |
dc.contributor.author | W J Choi | - |
dc.date.accessioned | 2017-04-19T08:59:38Z | - |
dc.date.available | 2017-04-19T08:59:38Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/6042 | - |
dc.description.abstract | It has been proposed that chitin synthase 3 (CHS3)-mediated chitin synthesis during the vegetative cell cycle is regulated by chitin synthase 4 (CHS4) of Saccharomyces cerevisiae. To investigate direct protein-protein interaction between the coding products of these two genes, a domain of Chs3p that is responsible for interaction with Chs4p was identified, using the yeast two-hybrid system. This domain of 54 amino acids, termed MIRC3-4 (Maximum Interacting Region of Chs3p with Chs4p), is well conserved among CHS3 homologs of various fungi. Some mutations in MIRC3-4 resulted in a decrease in the enzymatic activity and chitin contents. Chs3p carrying those mutations exhibited weak interactions with Chs4p, when assayed by the yeast two-hybrid system. Surprisingly, all the mutants were sensitive to Calcofluor regardless of changes in enzymatic activities or chitin contents. This report deals with a core region in MIRC3-4 that affects the interaction with Chs4p. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Identification of a domain in yeast chitin synthase 3 interacting with chitin synthase 4 by two-hybrid analysis | - |
dc.title.alternative | Identification of a domain in yeast chitin synthase 3 interacting with chitin synthase 4 by two-hybrid analysis | - |
dc.type | Article | - |
dc.citation.title | Journal of Microbiology and Biotechnology | - |
dc.citation.number | 6 | - |
dc.citation.endPage | 949 | - |
dc.citation.startPage | 943 | - |
dc.citation.volume | 12 | - |
dc.contributor.affiliatedAuthor | Sung Uk Kim | - |
dc.contributor.alternativeName | 박현숙 | - |
dc.contributor.alternativeName | 최신정 | - |
dc.contributor.alternativeName | 박녹현 | - |
dc.contributor.alternativeName | 김치화 | - |
dc.contributor.alternativeName | 김성욱 | - |
dc.contributor.alternativeName | 최원자 | - |
dc.identifier.bibliographicCitation | Journal of Microbiology and Biotechnology, vol. 12, no. 6, pp. 943-949 | - |
dc.subject.keyword | chitin synthase 3 | - |
dc.subject.keyword | saccharomyces cerevisiae | - |
dc.subject.keyword | ScCHS4 | - |
dc.subject.keyword | two-hybrid analysis | - |
dc.subject.local | chitin synthase 3 | - |
dc.subject.local | chitin synthase Ⅲ | - |
dc.subject.local | Saccharomyces cerevisiae | - |
dc.subject.local | saccharomyces cerevisiae | - |
dc.subject.local | ScCHS4 | - |
dc.subject.local | two-hybrid analysis | - |
dc.description.journalClass | Y | - |
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