Identification of a domain in yeast chitin synthase 3 interacting with chitin synthase 4 by two-hybrid analysis

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dc.contributor.authorH S Park-
dc.contributor.authorS J Choi-
dc.contributor.authorN H Park-
dc.contributor.authorC H Kim-
dc.contributor.authorSung Uk Kim-
dc.contributor.authorW J Choi-
dc.date.accessioned2017-04-19T08:59:38Z-
dc.date.available2017-04-19T08:59:38Z-
dc.date.issued2002-
dc.identifier.issn10177825-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6042-
dc.description.abstractIt has been proposed that chitin synthase 3 (CHS3)-mediated chitin synthesis during the vegetative cell cycle is regulated by chitin synthase 4 (CHS4) of Saccharomyces cerevisiae. To investigate direct protein-protein interaction between the coding products of these two genes, a domain of Chs3p that is responsible for interaction with Chs4p was identified, using the yeast two-hybrid system. This domain of 54 amino acids, termed MIRC3-4 (Maximum Interacting Region of Chs3p with Chs4p), is well conserved among CHS3 homologs of various fungi. Some mutations in MIRC3-4 resulted in a decrease in the enzymatic activity and chitin contents. Chs3p carrying those mutations exhibited weak interactions with Chs4p, when assayed by the yeast two-hybrid system. Surprisingly, all the mutants were sensitive to Calcofluor regardless of changes in enzymatic activities or chitin contents. This report deals with a core region in MIRC3-4 that affects the interaction with Chs4p.-
dc.publisherSouth Korea-
dc.titleIdentification of a domain in yeast chitin synthase 3 interacting with chitin synthase 4 by two-hybrid analysis-
dc.title.alternativeIdentification of a domain in yeast chitin synthase 3 interacting with chitin synthase 4 by two-hybrid analysis-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number6-
dc.citation.endPage949-
dc.citation.startPage943-
dc.citation.volume12-
dc.contributor.alternativeName박현숙-
dc.contributor.alternativeName최신정-
dc.contributor.alternativeName박녹현-
dc.contributor.alternativeName김치화-
dc.contributor.alternativeName김성욱-
dc.contributor.alternativeName최원자-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 12, no. 6, pp. 943-949-
dc.subject.keywordchitin synthase 3-
dc.subject.keywordsaccharomyces cerevisiae-
dc.subject.keywordScCHS4-
dc.subject.keywordtwo-hybrid analysis-
dc.subject.localchitin synthase 3-
dc.subject.localsaccharomyces cerevisiae-
dc.subject.localSaccharomyces cerevisiae-
dc.subject.localScCHS4-
dc.subject.localtwo-hybrid analysis-
dc.description.journalClassY-
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