Low-dose gamma irradiation as means of isolating carotenoid-hyperproducing yeast mutant

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dc.contributor.authorN K Sun-
dc.contributor.authorS H Lee-
dc.contributor.authorG H Ahn-
dc.contributor.authorMi Sun Won-
dc.contributor.authorK B Song-
dc.date.accessioned2017-04-19T08:59:38Z-
dc.date.available2017-04-19T08:59:38Z-
dc.date.issued2002-
dc.identifier.issn10177825-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6043-
dc.description.abstractIn order to isolate carotenoid-hyperproducing yeast, low-dose gamma irradiation was used as means of mutagenesis. Phaffia rhodozyma was treated by gamma irradiation of less than 10 kGy, which is considered to be a wholesome irradiation condition established by the Food and Drug Administration. Through repeated rounds of gamma irradiation and visual screening, mutant 3A4-8 was obtained. It produced a 3,824 μg carotenoid/g yeast, 69% higher content than 2,265 μg/g yeast of the unirradiated one. This result indicates that low-dose gamma irradiation could be used as means of mutagenesis to obtain carotenoid-hyperproducing strain of Phaffia rhodozyma, since only carotenoid-hyperproducing yeast survived gamma irradiation by scavenging oxygen radicals generated by radiolysis of water.-
dc.publisherSouth Korea-
dc.titleLow-dose gamma irradiation as means of isolating carotenoid-hyperproducing yeast mutant-
dc.title.alternativeLow-dose gamma irradiation as means of isolating carotenoid-hyperproducing yeast mutant-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number6-
dc.citation.endPage1012-
dc.citation.startPage1010-
dc.citation.volume12-
dc.contributor.affiliatedAuthorMi Sun Won-
dc.contributor.alternativeName선남규-
dc.contributor.alternativeName이승희-
dc.contributor.alternativeName안길환-
dc.contributor.alternativeName원미선-
dc.contributor.alternativeName송경빈-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 12, no. 6, pp. 1010-1012-
dc.subject.keywordγ-irradiation-
dc.subject.keywordcarotenoid-
dc.subject.keywordmutant-
dc.subject.localγ-irradiation-
dc.subject.localcarotenoid-
dc.subject.localCarotenoid-
dc.subject.localmutant-
dc.subject.localMutant-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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