Characterization of extracellular α-galactosidase produced by Streptomyces sp. YB-4 = 균체외 α-galactosidase를 생산하는 Streptomyces sp. YB-4의 분리 및 효소 특성

Abstract

A strain YB-4 producing the extracellular α-galactosidase was isolated from soil, and has been identified as Streptomyces sp. on the basis of its cultural, morphological and physiological properties. The partially purified α-galactosidase was most active on paranitrophenyl-α -D-galactopyranoside at pH 6.0 and 60°C. The enzyme retained 90% of its maximum activity between pH 4.0 and pH 10.0 after pre-incubation for 1 h. The enzyme was able to hydrolyze oligomeric substrates such as melibiose, raffinose and stachyose to liberate galactose residue, indicating that the α-galactosidase of Steptomyces sp. YB-4 hydrolyzed α-1,6 linkage.