NDP kinase 2 interacts with two oxidative stress-activated MAPKs to regulate cellular redox state and enhances multiple stress tolerance in transgenic plants

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dc.contributor.authorH J Moon-
dc.contributor.authorB Y Lee-
dc.contributor.authorG T Choi-
dc.contributor.authorD J Shin-
dc.contributor.authorD T Prasad-
dc.contributor.authorOk Sun Lee-
dc.contributor.authorSang Soo Kwak-
dc.contributor.authorD H Kim-
dc.contributor.authorJ S Nam-
dc.contributor.authorJ D Bahk-
dc.contributor.authorJ C Hong-
dc.contributor.authorS Y Lee-
dc.contributor.authorM J Cho-
dc.contributor.authorC O Lim-
dc.contributor.authorD J Yun-
dc.date.accessioned2017-04-19T08:59:40Z-
dc.date.available2017-04-19T08:59:40Z-
dc.date.issued2003-
dc.identifier.issn0027-8424-
dc.identifier.uri10.1073/pnas.252641899ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6053-
dc.description.abstractNDP kinases (NDPKs) are multifunctional proteins that regulate a variety of eukaryotic cellular activities, including cell proliferation, development, and differentiation. However, much less is known about the functional significance of NDPKs in plants. We show here that NDPK is associated with H2O2-mediated mitogen-activated protein kinase signaling in plants. H2O2 stress strongly induces the expression of the NDPK2 gene in Arabidopsis thaliana (AtNDPK2). Proteins from transgenic plants overexpressing AtNDPK2 showed high levels of autophosphorylation and NDPK activity, and they have lower levels of reactive oxygen species (ROS) than wild-type plants. Mutants lacking AtNDPK2 had higher levels of ROS than wild type. H2O2 treatment induced the phosphorylation of two endogenous proteins whose molecular weights suggested they are AtMPK3 and AtMPK6, two H2O2-activated A. thaliana mitogen-activated protein kinases. In the absence of H2O2 treatment, phosphorylation of these proteins was slightly elevated in plants overexpressing AtNDPK2 but markedly decreased in the AtNDPK2 deletion mutant. Yeast two-hybrid and in vitro protein pull-down assays revealed that AtNDPK2 specifically interacts with AtMPK3 and AtMPK6. Furthermore, AtNDPK2 also enhances the myelin basic protein phosphorylation activity of AtMPK3 in vitro. Finally, constitutive overexpression of AtNDPK2 in Arabidopsis plants conferred an enhanced tolerance to multiple environmental stresses that elicit ROS accumulation in situ. Thus, AtNDPK2 appears to play a previously uncharacterized regulatory role in H2O2-mediated MAPK signaling in plants.-
dc.publisherNatl Acad Sciences-
dc.titleNDP kinase 2 interacts with two oxidative stress-activated MAPKs to regulate cellular redox state and enhances multiple stress tolerance in transgenic plants-
dc.title.alternativeNDP kinase 2 interacts with two oxidative stress-activated MAPKs to regulate cellular redox state and enhances multiple stress tolerance in transgenic plants-
dc.typeArticle-
dc.citation.titleProceedings of National Academy of Sciences of United States of America-
dc.citation.number1-
dc.citation.endPage363-
dc.citation.startPage358-
dc.citation.volume100-
dc.contributor.affiliatedAuthorOk Sun Lee-
dc.contributor.affiliatedAuthorSang Soo Kwak-
dc.contributor.alternativeName문해정-
dc.contributor.alternativeName이보영-
dc.contributor.alternativeName최길추-
dc.contributor.alternativeName신동진-
dc.contributor.alternativeNamePrasad-
dc.contributor.alternativeName이옥선-
dc.contributor.alternativeName곽상수-
dc.contributor.alternativeName김도훈-
dc.contributor.alternativeName남재성-
dc.contributor.alternativeName박정동-
dc.contributor.alternativeName홍종찬-
dc.contributor.alternativeName이상열-
dc.contributor.alternativeName조무제-
dc.contributor.alternativeName임채오-
dc.contributor.alternativeName윤대진-
dc.identifier.bibliographicCitationProceedings of National Academy of Sciences of United States of America, vol. 100, no. 1, pp. 358-363-
dc.identifier.doi10.1073/pnas.252641899-
dc.description.journalClassY-
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Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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