High frequency somatic embryogenesis and plant regeneration in zygotic embryo cultures of Japanese honeysuckle

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Title
High frequency somatic embryogenesis and plant regeneration in zygotic embryo cultures of Japanese honeysuckle
Author(s)
Suk Weon Kim; Seung Cheol Oh; Dong Su In; Jang Ryol Liu
Bibliographic Citation
Plant Cell Tissue and Organ Culture, vol. 72, no. 3, pp. 277-280
Publication Year
2003
Abstract
Japanese honeysuckle plant (Lonicera japonica Thunb.) is rich in iridoid secologanin and is a potentially useful model for the study of secologanin biosynthesis. Culture conditions for high frequency plant regeneration via somatic embryogenesis from zygotic embryo cultures and zygotic embryo-derived embryogenic cell suspension cultures of this species are described. Mature zygotic embryos formed embryogenic calluses at a frequency of 46.7% when cultured on Murashige and Skoog (MS) medium supplemented with 4.52 μM 2,4-dichloro-phenoxyacetic acid (2,4-D). Cell suspension cultures were established with embryogenic calluses using liquid MS medium with 4.52 μM 2,4-D. Upon plating onto MS basal medium, embryogenic cell suspension cultures produced numerous somatic embryos, which subsequently developed into plantlets at a frequency of 68%. Regenerated plantlets were transplanted to potting soil and grown to maturity in a greenhouse.
Keyword
CaprifoliaceaeEmbryogenic callusLonicera japonicaSomatic embryoCell suspension cultures
ISSN
0167-6857
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1022360115275
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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