Functional over-expression of the Stm1 protein, a G-protein-coupled receptor, in Schizosaccharomyces pombe

Cited 3 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorKyung Sook Chung-
dc.contributor.authorDong Uk Kim-
dc.contributor.authorSung Woo Ryoo-
dc.contributor.authorEun Jung Kang-
dc.contributor.authorMi Sun Won-
dc.contributor.authorLi La Kim-
dc.contributor.authorYoung Joo Jang-
dc.contributor.authorP J Maeng-
dc.contributor.authorS C Kim-
dc.contributor.authorHyang Sook Yoo-
dc.contributor.authorKwang Lae Hoe-
dc.date.accessioned2017-04-19T08:59:40Z-
dc.date.available2017-04-19T08:59:40Z-
dc.date.issued2003-
dc.identifier.issn01415492-
dc.identifier.uri10.1023/A:1022355102192ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6058-
dc.description.abstractWe report here the first functional over-expression of the Stm1 protein, a G-protein-coupled receptor with seven-trans-membrane spanning regions, in a homologous expression system without internal modification of the open reading frame of Stm1. The entire coding sequence, except for the termination codon followed by a C-terminal His6 tag, has been cloned into the pREP1 vector. The functionally active Stm1-His6 was over-expressed in Schizosaccharomyces pombe under the control of the nmt1 (no message in thiamine) promoter. The expression after induction was 120 times as much as that of control before induction and it gave ∼500 ng protein/2 × 107cells.-
dc.publisherSpringer-
dc.titleFunctional over-expression of the Stm1 protein, a G-protein-coupled receptor, in Schizosaccharomyces pombe-
dc.title.alternativeFunctional over-expression of the Stm1 protein, a G-protein-coupled receptor, in Schizosaccharomyces pombe-
dc.typeArticle-
dc.citation.titleBiotechnology Letters-
dc.citation.number3-
dc.citation.endPage272-
dc.citation.startPage267-
dc.citation.volume25-
dc.contributor.affiliatedAuthorKyung Sook Chung-
dc.contributor.affiliatedAuthorMi Sun Won-
dc.contributor.alternativeName정경숙-
dc.contributor.alternativeName김동욱-
dc.contributor.alternativeName유승우-
dc.contributor.alternativeName강은정-
dc.contributor.alternativeName원미선-
dc.contributor.alternativeName김리라-
dc.contributor.alternativeName장영주-
dc.contributor.alternativeName맹필재-
dc.contributor.alternativeName김세창-
dc.contributor.alternativeName유향숙-
dc.contributor.alternativeName허광래-
dc.identifier.bibliographicCitationBiotechnology Letters, vol. 25, no. 3, pp. 267-272-
dc.identifier.doi10.1023/A:1022355102192-
dc.subject.keywordG-protein-coupled receptor-
dc.subject.keywordHis6 tag-
dc.subject.keywordHomologous expression-
dc.subject.keywordSchizosaccharomyces pombe-
dc.subject.keywordSeven transmembrane protein-
dc.subject.localG-protein-coupled receptor-
dc.subject.localHis6 tag-
dc.subject.localHomologous expression-
dc.subject.localSchizosaccharomyces pombe-
dc.subject.localSeven transmembrane protein-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > 1. Journal Articles
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.