Molecular characterization of NbPAF encoding the α6 subunit of the 20S proteasome in Nicotiana benthamiana

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dc.contributor.authorMoonil Kim-
dc.contributor.authorKyoung Sil Yang-
dc.contributor.authorYu Kyung Kim-
dc.contributor.authorK H Paek-
dc.contributor.authorHyun Sook Pai-
dc.description.abstractThe 26S proteasome involved in degradation of proteins covalently modified with polyubiquitin consists of the 20S proteasome and 19S regulatory complex. The NbPAF gene encoding the α6 subunit of the 20S proteasome was identified from Nicotiana benthamiana. NbPAF exhibits high sequence homology with the corresponding genes from Arabidopsis, human and yeast. The deduced amino acid sequence of NbPAF reveals that this protein contains the proteasome α-type subunits signature and nuclear localization signal at the N-terminus. The genomic Southern blot analysis suggests that the N. benthamiana genome contains one copy of NbPAF. The NbPAF mRNA was detected abundantly in flowers and weakly in roots and stems, but it was almost undetectable in mature leaves. In response to stresses, accumulation of the NbPAF mRNA was stimulated by methyl jasmonate, NaCl and salicylic acid, but not by abscisic acid and cold treatment in leaves. The NbPAF-GFP fusion protein was localized in the cytoplasm and nucleus.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleMolecular characterization of NbPAF encoding the α6 subunit of the 20S proteasome in Nicotiana benthamiana-
dc.title.alternativeMolecular characterization of NbPAF encoding the α6 subunit of the 20S proteasome in Nicotiana benthamiana-
dc.citation.titleMolecules and Cells-
dc.contributor.affiliatedAuthorMoonil Kim-
dc.contributor.affiliatedAuthorKyoung Sil Yang-
dc.contributor.affiliatedAuthorYu Kyung Kim-
dc.contributor.affiliatedAuthorHyun Sook Pai-
dc.identifier.bibliographicCitationMolecules and Cells, vol. 15, no. 1, pp. 127-132-
dc.subject.keywordinducible expression-
dc.subject.keywordplant defense response-
dc.subject.keywordsingle gene-
dc.subject.keywordsubcellular localization-
dc.subject.localInducible expression-
dc.subject.localinducible expression-
dc.subject.localPlant defense responses-
dc.subject.localplant defense response-
dc.subject.localsingle gene-
dc.subject.localSingle gene-
dc.subject.localsubcellular localization-
dc.subject.localSubcellular localization-
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